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10.1080/15476286.2021.1926648 http://scihub22266oqcxt.onion/10.1080/15476286.2021.1926648 33966602!8174584!33966602     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA+Biol 2021 ; 18 (12): 2218-2225 Nephropedia Template TP {{tp|p=33966602|t=2021. A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2 |pdf=|usr=}}{{33966602}} gab.com Text A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2 JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=33966602 #FOAvip Early detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proven crucial during the efforts to mitigate the effects of the COVID-19 pandemic. Several diagnostic methods have emerged in the past few months, each with different shortcomings and limitations. The current gold standard, RT-qPCR using fluorescent probes, relies on demanding equipment requirements plus the high costs of the probes and specific reaction mixes. To broaden the possibilities of reagents and thermocyclers that could be allocated towards this task, we have optimized an alternative strategy for RT-qPCR diagnosis. This is based on a widely used DNA-intercalating dye and can be implemented with several different qPCR reagents and instruments. Remarkably, the proposed qPCR method performs similarly to the broadly used TaqMan-based detection, in terms of specificity and sensitivity, thus representing a reliable tool. We think that, through enabling the use of vast range of thermocycler models and laboratory facilities for SARS-CoV-2 diagnosis, the alternative proposed here can increase dramatically the testing capability, especially in countries with limited access to costly technology and reagents. Twit Text FOAVip A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2 ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=33966602 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33966602 #FOAvip English Wikipedia <ref>{{Cite pmid|33966602}}</ref> A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2 #MMPMID33966602 Fuchs Wightman F; Godoy Herz MA; Munoz JC; Stigliano JN; Bragado L; Moreno NN; Palavecino M; Servi L; Cabrerizo G; Clemente J; Avaro M; Pontoriero A; Benedetti E; Baumeister E; Rudolf F; Remes Lenicov F; Garcia C; Buggiano V; Kornblihtt AR; Srebrow A; de la Mata M; Munoz MJ; Schor IE; Petrillo E RNA Biol 2021[Dec]; 18 (12): 2218-2225 PMID33966602show ga Early detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been proven crucial during the efforts to mitigate the effects of the COVID-19 pandemic. Several diagnostic methods have emerged in the past few months, each with different shortcomings and limitations. The current gold standard, RT-qPCR using fluorescent probes, relies on demanding equipment requirements plus the high costs of the probes and specific reaction mixes. To broaden the possibilities of reagents and thermocyclers that could be allocated towards this task, we have optimized an alternative strategy for RT-qPCR diagnosis. This is based on a widely used DNA-intercalating dye and can be implemented with several different qPCR reagents and instruments. Remarkably, the proposed qPCR method performs similarly to the broadly used TaqMan-based detection, in terms of specificity and sensitivity, thus representing a reliable tool. We think that, through enabling the use of vast range of thermocycler models and laboratory facilities for SARS-CoV-2 diagnosis, the alternative proposed here can increase dramatically the testing capability, especially in countries with limited access to costly technology and reagents. |Benzothiazoles/*chemistry[MESH] |COVID-19 Nucleic Acid Testing/*methods/standards[MESH] |COVID-19/*diagnosis/virology[MESH] |DNA Primers/chemistry/metabolism[MESH] |DNA/analysis/biosynthesis[MESH] |Diamines/*chemistry[MESH] |Humans[MESH] |Intercalating Agents/*chemistry[MESH] |Nasopharynx/virology[MESH] |Quinolines/*chemistry[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods/standards[MESH] |SARS-CoV-2/*genetics[MESH]A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV(epmc).pdf DeepDyve Pubget Overpricing