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10.12122/j.issn.1673-4254.2021.04.01 http://scihub22266oqcxt.onion/10.12122/j.issn.1673-4254.2021.04.01 33963705!8110440!33963705     free     free     free Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538 Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538       Nan+Fang+Yi+Ke+Da+Xue+Xue+Bao 2021 ; 41 (4): 475-482 Nephropedia Template TP {{tp|p=33963705|t=2021. Salvianolic acid B and its magnesium salt inhibit SARS-CoV-2 infection of Vero-E6 cells by blocking spike protein-mediated membrane fusion |pdf=|usr=}}{{33963705}} gab.com Text Salvianolic acid B and its magnesium salt inhibit SARS-CoV-2 infection of Vero-E6 cells by blocking spike protein-mediated membrane fusion JO: Nan Fang Yi Ke Da Xue Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=33963705 #FOAvip OBJECTIVE: The investigate the inhibitory effects of the traditional Chinese medicine (TCM) monomer salvianolic acid B (Sal-B) and its magnesium salt Salvia Miltiorrhiza Polyphenolate Injection (ZDDY) against SARS-CoV-2 infection in vitro and explore the molecular mechanism. OBJECTIVE: The anti-SARS-CoV-2 activity of Sal-B and ZDDY was assessed using the authentic and pseudotyped SARS-CoV-2 infection assay. The antiviral targets of Sal-B were identified by molecular docking and molecular dynamics simulation. Circular dichroism spectroscopy was used to examine the structural characteristics of HR1 and HR2 regions of SARS-CoV-2 S protein, and the S protein-mediated cell-cell fusion assay was used to evaluate the effect of Sal-B on virus-cell membrane fusion. Flow cytometry was carried out to analyze the effect of Sal-B on the binding of SARS-CoV-2 RBD to hACE2 receptor. OBJECTIVE: The median effective concentrations (EC(50)) of Sal-B and ZDDY against SARSCoV-2 infection in Vero-E6 cells were 55.47 mumol/L and 36.07 mug/mL, respectively. Both Sal-B and ZDDY successfully inhibited the entry of SARS-CoV-2 pseudovirus into the cells that stably expressed human ACE2 (ACE2/293T), with half maximal inhibitory concentrations (IC(50)) of 1.69 mumol/L and 24.81 mug/mL, respectively. Sal-B showed a binding affinity of -8.2 kcal/mol to the 6-helix bundle (6-HB) of SARS-CoV-2 S protein. Molecular dynamics simulation showed stable binding between Sal-B and the 6-HB of SARS-CoV-2 S protein at the predicted binding site. Sal-B disturbed the formation of the secondary structure of 6-HB in HR1P/HR2P mixture, resulting in a significantly lowered alpha-helicity (P < 0.05). Sal-B dose-dependently inhibited SARS-CoV-2 S protein-mediated cell-cell fusion, with an IC(50) of 3.33 mumol/L. Sal-B showed no effect on RBD-Fc protein binding to the ACE2 receptor. OBJECTIVE: Sal-B and its magnesium salt ZDDY can inhibit the entry of SARS-CoV-2 in Vero-E6 cells in vitro by blocking SARS-CoV-2 spike protein-mediated virus-cell membrane fusion. Twit Text FOAVip Salvianolic acid B and its magnesium salt inhibit SARS-CoV-2 infection of Vero-E6 cells by blocking spike protein-mediated membrane fusion ????Nan Fang Yi Ke Da Xue Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=33963705 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33963705 #FOAvip English Wikipedia <ref>{{Cite pmid|33963705}}</ref> Salvianolic acid B and its magnesium salt inhibit SARS-CoV-2 infection of Vero-E6 cells by blocking spike protein-mediated membrane fusion #MMPMID33963705 Yang C; Cheng C; Wang J; Chen K; Zhan J; Pan X; Xu X; Xu W; Liu S Nan Fang Yi Ke Da Xue Xue Bao 2021[Apr]; 41 (4): 475-482 PMID33963705show ga OBJECTIVE: The investigate the inhibitory effects of the traditional Chinese medicine (TCM) monomer salvianolic acid B (Sal-B) and its magnesium salt Salvia Miltiorrhiza Polyphenolate Injection (ZDDY) against SARS-CoV-2 infection in vitro and explore the molecular mechanism. OBJECTIVE: The anti-SARS-CoV-2 activity of Sal-B and ZDDY was assessed using the authentic and pseudotyped SARS-CoV-2 infection assay. The antiviral targets of Sal-B were identified by molecular docking and molecular dynamics simulation. Circular dichroism spectroscopy was used to examine the structural characteristics of HR1 and HR2 regions of SARS-CoV-2 S protein, and the S protein-mediated cell-cell fusion assay was used to evaluate the effect of Sal-B on virus-cell membrane fusion. Flow cytometry was carried out to analyze the effect of Sal-B on the binding of SARS-CoV-2 RBD to hACE2 receptor. OBJECTIVE: The median effective concentrations (EC(50)) of Sal-B and ZDDY against SARSCoV-2 infection in Vero-E6 cells were 55.47 mumol/L and 36.07 mug/mL, respectively. Both Sal-B and ZDDY successfully inhibited the entry of SARS-CoV-2 pseudovirus into the cells that stably expressed human ACE2 (ACE2/293T), with half maximal inhibitory concentrations (IC(50)) of 1.69 mumol/L and 24.81 mug/mL, respectively. Sal-B showed a binding affinity of -8.2 kcal/mol to the 6-helix bundle (6-HB) of SARS-CoV-2 S protein. Molecular dynamics simulation showed stable binding between Sal-B and the 6-HB of SARS-CoV-2 S protein at the predicted binding site. Sal-B disturbed the formation of the secondary structure of 6-HB in HR1P/HR2P mixture, resulting in a significantly lowered alpha-helicity (P < 0.05). Sal-B dose-dependently inhibited SARS-CoV-2 S protein-mediated cell-cell fusion, with an IC(50) of 3.33 mumol/L. Sal-B showed no effect on RBD-Fc protein binding to the ACE2 receptor. OBJECTIVE: Sal-B and its magnesium salt ZDDY can inhibit the entry of SARS-CoV-2 in Vero-E6 cells in vitro by blocking SARS-CoV-2 spike protein-mediated virus-cell membrane fusion. |*COVID-19[MESH] |*Spike Glycoprotein, Coronavirus/metabolism[MESH] |Animals[MESH] |Benzofurans[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Magnesium[MESH] |Membrane Fusion[MESH] |Molecular Docking Simulation[MESH] |Protein Binding[MESH] Salvianolic acid B and its magnesium salt inhibit SARS-CoV-2 infectio(epmc).pdf DeepDyve Pubget Overpricing