Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1128/JVI.00663-21 http://scihub22266oqcxt.onion/10.1128/JVI.00663-21 33963053!8223950!33963053     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol 2021 ; 95 (14): e0066321 Nephropedia Template TP {{tp|p=33963053|t=2021. Targeting the Conserved Stem Loop 2 Motif in the SARS-CoV-2 Genome |pdf=|usr=}}{{33963053}} gab.com Text Targeting the Conserved Stem Loop 2 Motif in the SARS-CoV-2 Genome JO: J Virol http://www.kidney.de/pget.php?&tw=1&pmid=33963053 #FOAvip RNA structural elements occur in numerous single-stranded positive-sense RNA viruses. The stem-loop 2 motif (s2m) is one such element with an unusually high degree of sequence conservation, being found in the 3' untranslated region (UTR) in the genomes of many astroviruses, some picornaviruses and noroviruses, and a variety of coronaviruses, including severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. The evolutionary conservation and its occurrence in all viral subgenomic transcripts imply a key role for s2m in the viral infection cycle. Our findings indicate that the element, while stably folded, can nonetheless be invaded and remodeled spontaneously by antisense oligonucleotides (ASOs) that initiate pairing in exposed loops and trigger efficient sequence-specific RNA cleavage in reporter assays. ASOs also act to inhibit replication in an astrovirus replicon model system in a sequence-specific, dose-dependent manner and inhibit SARS-CoV-2 replication in cell culture. Our results thus permit us to suggest that the s2m element is readily targeted by ASOs, which show promise as antiviral agents. IMPORTANCE The highly conserved stem-loop 2 motif (s2m) is found in the genomes of many RNA viruses, including SARS-CoV-2. Our findings indicate that the s2m element can be targeted by antisense oligonucleotides. The antiviral potential of this element represents a promising start for further research into targeting conserved elements in RNA viruses. Twit Text FOAVip Targeting the Conserved Stem Loop 2 Motif in the SARS-CoV-2 Genome ????J Virol http://www.kidney.de/pget.php?&tw=1&pmid=33963053 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33963053 #FOAvip English Wikipedia <ref>{{Cite pmid|33963053}}</ref> Targeting the Conserved Stem Loop 2 Motif in the SARS-CoV-2 Genome #MMPMID33963053 Lulla V; Wandel MP; Bandyra KJ; Ulferts R; Wu M; Dendooven T; Yang X; Doyle N; Oerum S; Beale R; O'Rourke SM; Randow F; Maier HJ; Scott W; Ding Y; Firth AE; Bloznelyte K; Luisi BF J Virol 2021[Jun]; 95 (14): e0066321 PMID33963053show ga RNA structural elements occur in numerous single-stranded positive-sense RNA viruses. The stem-loop 2 motif (s2m) is one such element with an unusually high degree of sequence conservation, being found in the 3' untranslated region (UTR) in the genomes of many astroviruses, some picornaviruses and noroviruses, and a variety of coronaviruses, including severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. The evolutionary conservation and its occurrence in all viral subgenomic transcripts imply a key role for s2m in the viral infection cycle. Our findings indicate that the element, while stably folded, can nonetheless be invaded and remodeled spontaneously by antisense oligonucleotides (ASOs) that initiate pairing in exposed loops and trigger efficient sequence-specific RNA cleavage in reporter assays. ASOs also act to inhibit replication in an astrovirus replicon model system in a sequence-specific, dose-dependent manner and inhibit SARS-CoV-2 replication in cell culture. Our results thus permit us to suggest that the s2m element is readily targeted by ASOs, which show promise as antiviral agents. IMPORTANCE The highly conserved stem-loop 2 motif (s2m) is found in the genomes of many RNA viruses, including SARS-CoV-2. Our findings indicate that the s2m element can be targeted by antisense oligonucleotides. The antiviral potential of this element represents a promising start for further research into targeting conserved elements in RNA viruses. |*COVID-19/genetics/metabolism[MESH] |*Genome, Viral[MESH] |*Nucleotide Motifs[MESH] |*RNA Folding[MESH] |*RNA, Viral/genetics/metabolism[MESH] |*Virus Replication[MESH] |Animals[MESH] |Chlorocebus aethiops[MESH] |HEK293 Cells[MESH] |Humans[MESH] |SARS-CoV-2/*physiology[MESH]Targeting the Conserved Stem Loop 2 Motif in the SARS-CoV-2 Genome (epmc).pdf DeepDyve Pubget Overpricing