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10.1016/j.celrep.2021.109108 http://scihub22266oqcxt.onion/10.1016/j.celrep.2021.109108 33961822!8062406!33961822     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2021 ; 35 (6): 109108 Nephropedia Template TP {{tp|p=33961822|t=2021. Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection |pdf=|usr=}}{{33961822}} gab.com Text Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=33961822 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses subgenomic RNA (sgRNA) to produce viral proteins for replication and immune evasion. We apply long-read RNA and cDNA sequencing to in vitro human and primate infection models to study transcriptional dynamics. Transcription-regulating sequence (TRS)-dependent sgRNA upregulates earlier in infection than TRS-independent sgRNA. An abundant class of TRS-independent sgRNA consisting of a portion of open reading frame 1ab (ORF1ab) containing nsp1 joins to ORF10, and the 3' untranslated region (UTR) upregulates at 48 h post-infection in human cell lines. We identify double-junction sgRNA containing both TRS-dependent and -independent junctions. We find multiple sites at which the SARS-CoV-2 genome is consistently more modified than sgRNA and that sgRNA modifications are stable across transcript clusters, host cells, and time since infection. Our work highlights the dynamic nature of the SARS-CoV-2 transcriptome during its replication cycle. Twit Text FOAVip Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=33961822 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33961822 #FOAvip English Wikipedia <ref>{{Cite pmid|33961822}}</ref> Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during cellular infection #MMPMID33961822 Chang JJ; Rawlinson D; Pitt ME; Taiaroa G; Gleeson J; Zhou C; Mordant FL; De Paoli-Iseppi R; Caly L; Purcell DFJ; Stinear TP; Londrigan SL; Clark MB; Williamson DA; Subbarao K; Coin LJM Cell Rep 2021[May]; 35 (6): 109108 PMID33961822show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses subgenomic RNA (sgRNA) to produce viral proteins for replication and immune evasion. We apply long-read RNA and cDNA sequencing to in vitro human and primate infection models to study transcriptional dynamics. Transcription-regulating sequence (TRS)-dependent sgRNA upregulates earlier in infection than TRS-independent sgRNA. An abundant class of TRS-independent sgRNA consisting of a portion of open reading frame 1ab (ORF1ab) containing nsp1 joins to ORF10, and the 3' untranslated region (UTR) upregulates at 48 h post-infection in human cell lines. We identify double-junction sgRNA containing both TRS-dependent and -independent junctions. We find multiple sites at which the SARS-CoV-2 genome is consistently more modified than sgRNA and that sgRNA modifications are stable across transcript clusters, host cells, and time since infection. Our work highlights the dynamic nature of the SARS-CoV-2 transcriptome during its replication cycle. |Animals[MESH] |COVID-19/*genetics[MESH] |Caco-2 Cells[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Epigenesis, Genetic[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |Immune Evasion[MESH] |Open Reading Frames[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics[MESH] |Transcription, Genetic/*genetics[MESH] |Transcriptome[MESH] |Vero Cells[MESH]Transcriptional and epi-transcriptional dynamics of SARS-CoV-2 during (epmc).pdf DeepDyve Pubget Overpricing