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10.1371/journal.pbio.3001236 http://scihub22266oqcxt.onion/10.1371/journal.pbio.3001236 33961632!8133773!33961632     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Biol 2021 ; 19 (5): e3001236 Nephropedia Template TP {{tp|p=33961632|t=2021. Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2 |pdf=|usr=}}{{33961632}} gab.com Text Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2 JO: PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=33961632 #FOAvip With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages. It was found that the B.1.1.7 (also 501Y.V1) variant, first detected in the United Kingdom, could be serendipitously detected by the Thermo Fisher TaqPath COVID-19 PCR assay because a key deletion in these viruses, spike Delta69-70, would cause a "spike gene target failure" (SGTF) result. However, a SGTF result is not definitive for B.1.1.7, and this assay cannot detect other variants of concern (VOC) that lack spike Delta69-70, such as B.1.351 (also 501Y.V2), detected in South Africa, and P.1 (also 501Y.V3), recently detected in Brazil. We identified a deletion in the ORF1a gene (ORF1a Delta3675-3677) in all 3 variants, which has not yet been widely detected in other SARS-CoV-2 lineages. Using ORF1a Delta3675-3677 as the primary target and spike Delta69-70 to differentiate, we designed and validated an open-source PCR assay to detect SARS-CoV-2 VOC. Our assay can be rapidly deployed in laboratories around the world to enhance surveillance for the local emergence and spread of B.1.1.7, B.1.351, and P.1. Twit Text FOAVip Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2 ????PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=33961632 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33961632 #FOAvip English Wikipedia <ref>{{Cite pmid|33961632}}</ref> Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2 #MMPMID33961632 Vogels CBF; Breban MI; Ott IM; Alpert T; Petrone ME; Watkins AE; Kalinich CC; Earnest R; Rothman JE; Goes de Jesus J; Morales Claro I; Magalhaes Ferreira G; Crispim MAE; Singh L; Tegally H; Anyaneji UJ; Hodcroft EB; Mason CE; Khullar G; Metti J; Dudley JT; MacKay MJ; Nash M; Wang J; Liu C; Hui P; Murphy S; Neal C; Laszlo E; Landry ML; Muyombwe A; Downing R; Razeq J; de Oliveira T; Faria NR; Sabino EC; Neher RA; Fauver JR; Grubaugh ND PLoS Biol 2021[May]; 19 (5): e3001236 PMID33961632show ga With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages. It was found that the B.1.1.7 (also 501Y.V1) variant, first detected in the United Kingdom, could be serendipitously detected by the Thermo Fisher TaqPath COVID-19 PCR assay because a key deletion in these viruses, spike Delta69-70, would cause a "spike gene target failure" (SGTF) result. However, a SGTF result is not definitive for B.1.1.7, and this assay cannot detect other variants of concern (VOC) that lack spike Delta69-70, such as B.1.351 (also 501Y.V2), detected in South Africa, and P.1 (also 501Y.V3), recently detected in Brazil. We identified a deletion in the ORF1a gene (ORF1a Delta3675-3677) in all 3 variants, which has not yet been widely detected in other SARS-CoV-2 lineages. Using ORF1a Delta3675-3677 as the primary target and spike Delta69-70 to differentiate, we designed and validated an open-source PCR assay to detect SARS-CoV-2 VOC. Our assay can be rapidly deployed in laboratories around the world to enhance surveillance for the local emergence and spread of B.1.1.7, B.1.351, and P.1. |COVID-19/diagnosis/genetics/*virology[MESH] |DNA Primers[MESH] |Humans[MESH] |Multiplex Polymerase Chain Reaction/methods[MESH] |Mutation[MESH] |Polyproteins/genetics[MESH] |SARS-CoV-2/*genetics[MESH]Multiplex qPCR discriminates variants of concern to enhance global sur(epmc).pdf DeepDyve Pubget Overpricing