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10.1021/acs.analchem.1c00677 http://scihub22266oqcxt.onion/10.1021/acs.analchem.1c00677 33955213!ä!33955213 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chem 2021 ; 93 (19): 7283-7291 Nephropedia Template TP {{tp|p=33955213|t=2021. Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid Protein |pdf=|usr=}}{{33955213}} gab.com Text Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid Protein JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33955213 #FOAvip The goal of this work was to develop recombinantly expressed variable domains derived from camelid heavy-chain antibodies known as single-domain antibodies (sdAbs) directed against the SARS-CoV-2 nucleocapsid protein for incorporation into detection assays. To achieve this, a llama was immunized using a recombinant SARS-CoV-2 nucleocapsid protein and an immune phage-display library of variable domains was developed. The sdAbs selected from this library segregated into five distinct sequence families. Three of these families bind to unique epitopes with high affinity, low nM to sub-nM K(D), as determined by surface plasmon resonance. To further enhance the utility of these sdAbs for the detection of nucleocapsid protein, homobivalent and heterobivalent genetic fusion constructs of the three high-affinity sdAbs were prepared. The effectiveness of the sdAbs for the detection of nucleocapsid protein was evaluated using MagPlex fluid array assays, a multiplexed immunoassay on color-coded magnetic microspheres. Using the optimal bivalent pair, one immobilized on the microsphere and the other serving as the biotinylated recognition reagent, a detection limit as low as 50 pg/mL of recombinant nucleocapsid and of killed virus down to 1.28 x 10(3) pfu/mL was achieved. The sdAbs described here represent immune reagents that can be tailored to be optimized for a number of detection platforms and may one day aid in the detection of SARS-CoV-2 to assist in controlling the current pandemic. Twit Text FOAVip Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid Protein ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33955213 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33955213 #FOAvip English Wikipedia <ref>{{Cite pmid|33955213}}</ref> Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid Protein #MMPMID33955213 Anderson GP; Liu JL; Esparza TJ; Voelker BT; Hofmann ER; Goldman ER Anal Chem 2021[May]; 93 (19): 7283-7291 PMID33955213show ga The goal of this work was to develop recombinantly expressed variable domains derived from camelid heavy-chain antibodies known as single-domain antibodies (sdAbs) directed against the SARS-CoV-2 nucleocapsid protein for incorporation into detection assays. To achieve this, a llama was immunized using a recombinant SARS-CoV-2 nucleocapsid protein and an immune phage-display library of variable domains was developed. The sdAbs selected from this library segregated into five distinct sequence families. Three of these families bind to unique epitopes with high affinity, low nM to sub-nM K(D), as determined by surface plasmon resonance. To further enhance the utility of these sdAbs for the detection of nucleocapsid protein, homobivalent and heterobivalent genetic fusion constructs of the three high-affinity sdAbs were prepared. The effectiveness of the sdAbs for the detection of nucleocapsid protein was evaluated using MagPlex fluid array assays, a multiplexed immunoassay on color-coded magnetic microspheres. Using the optimal bivalent pair, one immobilized on the microsphere and the other serving as the biotinylated recognition reagent, a detection limit as low as 50 pg/mL of recombinant nucleocapsid and of killed virus down to 1.28 x 10(3) pfu/mL was achieved. The sdAbs described here represent immune reagents that can be tailored to be optimized for a number of detection platforms and may one day aid in the detection of SARS-CoV-2 to assist in controlling the current pandemic. |*COVID-19[MESH] |*Camelids, New World[MESH] |*Single-Domain Antibodies[MESH] |Animals[MESH] |Humans[MESH] |Nucleocapsid Proteins/genetics[MESH]Single-Domain Antibodies for the Detection of SARS-CoV-2 Nucleocapsid (epmc).pdf DeepDyve Pubget Overpricing