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10.1371/journal.pone.0250202 http://scihub22266oqcxt.onion/10.1371/journal.pone.0250202 33951060!8099103!33951060     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (5): e0250202 Nephropedia Template TP {{tp|p=33951060|t=2021. Low saliva pH can yield false positives results in simple RT-LAMP-based SARS-CoV-2 diagnostic tests |pdf=|usr=}}{{33951060}} gab.com Text Low saliva pH can yield false positives results in simple RT-LAMP-based SARS-CoV-2 diagnostic tests JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33951060 #FOAvip Diagnosis of any infectious disease is vital for opportune treatment and to prevent dissemination. RT-qPCR tests for detection of SARS-CoV-2, the causative agent for COVID-19, are ideal in a hospital environment. However, mass testing requires cheaper and simpler tests, especially in settings that lack sophisticated machinery. The most common current diagnostic method is based on nasopharyngeal sample collection, RNA extraction, and RT-qPCR for amplification and detection of viral nucleic acids. Here, we show that samples obtained from nasopharyngeal swabs in VTM and in saliva can be used with or without RNA purification in an isothermal loop-mediated amplification (LAMP)-based assay, with 60-93% sensitivity for SARS-CoV-2 detection as compared to standard RT-qPCR tests. A series of simple modifications to standard RT-LAMP published methods to stabilize pH fluctuations due to salivary acidity resulted in a significant improvement in reliability, opening new avenues for efficient, low-cost testing of COVID-19 infection. Twit Text FOAVip Low saliva pH can yield false positives results in simple RT-LAMP-based SARS-CoV-2 diagnostic tests ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33951060 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33951060 #FOAvip English Wikipedia <ref>{{Cite pmid|33951060}}</ref> Low saliva pH can yield false positives results in simple RT-LAMP-based SARS-CoV-2 diagnostic tests #MMPMID33951060 Uribe-Alvarez C; Lam Q; Baldwin DA; Chernoff J PLoS One 2021[]; 16 (5): e0250202 PMID33951060show ga Diagnosis of any infectious disease is vital for opportune treatment and to prevent dissemination. RT-qPCR tests for detection of SARS-CoV-2, the causative agent for COVID-19, are ideal in a hospital environment. However, mass testing requires cheaper and simpler tests, especially in settings that lack sophisticated machinery. The most common current diagnostic method is based on nasopharyngeal sample collection, RNA extraction, and RT-qPCR for amplification and detection of viral nucleic acids. Here, we show that samples obtained from nasopharyngeal swabs in VTM and in saliva can be used with or without RNA purification in an isothermal loop-mediated amplification (LAMP)-based assay, with 60-93% sensitivity for SARS-CoV-2 detection as compared to standard RT-qPCR tests. A series of simple modifications to standard RT-LAMP published methods to stabilize pH fluctuations due to salivary acidity resulted in a significant improvement in reliability, opening new avenues for efficient, low-cost testing of COVID-19 infection. |COVID-19/*diagnosis/virology[MESH] |False Positive Reactions[MESH] |Humans[MESH] |Hydrogen-Ion Concentration[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nasopharynx/virology[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |RNA, Viral/*analysis[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Saliva/*chemistry[MESH]Low saliva pH can yield false positives results in simple RT-LAMP-base(epmc).pdf DeepDyve Pubget Overpricing