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10.1039/d0cc07922g http://scihub22266oqcxt.onion/10.1039/d0cc07922g 33949414!?!33949414 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33949414&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Chem+Commun+(Camb) 2021 ; 57 (44): 5434-5437 Nephropedia Template TP {{tp|p=33949414|t=2021. Selenium-atom-modified thymidine enhances the specificity and sensitivity of DNA polymerization and detection |pdf=|usr=}}{{33949414}} gab.com Text Selenium-atom-modified thymidine enhances the specificity and sensitivity of DNA polymerization and detection JO: Chem Commun (Camb) http://www.kidney.de/pget.php?&tw=1&pmid=33949414 #FOAvip Nucleobase mismatches can jeopardize DNA polymerization specificity, causing mutations and errors in DNA replication and detection. Herein we report the first synthesis of novel 2-Se-thymidine triphosphate (SeTTP), describe the single-selenium atom-specific modification strategy (SAM) against T/G mismatches, and demonstrate SAM-assisted polymerization and detection with much higher specificity and sensitivity. SAM can effectively suppress the formation of non-specific products in DNA polymerization and detection. Thus, SAM enhances the specificity of DNA synthesis by approximately 10 000 fold, and in turn, it allows the detection of clinical COVID-19 viral RNA in low copy numbers (single-digit copies), while the conventional RT-qPCR does not. Twit Text FOAVip Selenium-atom-modified thymidine enhances the specificity and sensitivity of DNA polymerization and detection ????Chem Commun (Camb) http://www.kidney.de/pget.php?&tw=1&pmid=33949414 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33949414 #FOAvip English Wikipedia <ref>{{Cite pmid|33949414}}</ref> Selenium-atom-modified thymidine enhances the specificity and sensitivity of DNA polymerization and detection #MMPMID33949414 Li Y; Zhou Y; Luo D; Yang Z; Hu LR; Huang Z Chem Commun (Camb) 2021[Jun]; 57 (44): 5434-5437 PMID33949414show ga Nucleobase mismatches can jeopardize DNA polymerization specificity, causing mutations and errors in DNA replication and detection. Herein we report the first synthesis of novel 2-Se-thymidine triphosphate (SeTTP), describe the single-selenium atom-specific modification strategy (SAM) against T/G mismatches, and demonstrate SAM-assisted polymerization and detection with much higher specificity and sensitivity. SAM can effectively suppress the formation of non-specific products in DNA polymerization and detection. Thus, SAM enhances the specificity of DNA synthesis by approximately 10 000 fold, and in turn, it allows the detection of clinical COVID-19 viral RNA in low copy numbers (single-digit copies), while the conventional RT-qPCR does not. |*Polymerization[MESH] |DNA/*chemistry[MESH] |Selenium/*chemistry[MESH]Selenium-atom-modified thymidine enhances the specificity and sensitiv(epmc).pdf DeepDyve Pubget Overpricing