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10.1186/s12985-021-01567-3 http://scihub22266oqcxt.onion/10.1186/s12985-021-01567-3 33947425!8094981!33947425     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virol+J 2021 ; 18 (1): 95 Nephropedia Template TP {{tp|p=33947425|t=2021. Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR |pdf=|usr=}}{{33947425}} gab.com Text Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR JO: Virol J http://www.kidney.de/pget.php?&tw=1&pmid=33947425 #FOAvip The early identification of asymptomatic yet infectious cases is vital to curb the 2019 coronavirus (COVID-19) pandemic and to control the disease in the post-pandemic era. In this paper, we propose a fast, inexpensive and high-throughput approach using painless nasal-swab self-collection followed by direct RT-qPCR for the sensitive PCR detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This approach was validated in a large prospective cohort study of 1038 subjects, analysed simultaneously using (1) nasopharyngeal swabs obtained with the assistance of healthcare personnel and analysed by classic two-step RT-qPCR on RNA isolates and (2) nasal swabs obtained by self-collection and analysed with direct RT-qPCR. Of these subjects, 28.6% tested positive for SARS-CoV-2 using nasopharyngeal swab sampling. Our direct RT-qPCR approach for self-collected nasal swabs performed well with results similar to those of the two-step RT-qPCR on RNA isolates, achieving 0.99 positive and 0.98 negative predictive values (cycle threshold [Ct] < 37). Our research also reports on grey-zone viraemia, including samples with near-cut-off Ct values (Ct >/= 37). In all investigated subjects (n = 20) with grey-zone viraemia, the ultra-small viral load disappeared within hours or days with no symptoms. Overall, this study underscores the importance of painless nasal-swab self-collection and direct RT-qPCR for mass testing during the SARS-CoV-2 pandemic and in the post-pandemic era. Twit Text FOAVip Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR ????Virol J http://www.kidney.de/pget.php?&tw=1&pmid=33947425 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33947425 #FOAvip English Wikipedia <ref>{{Cite pmid|33947425}}</ref> Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR #MMPMID33947425 Kriegova E; Fillerova R; Raska M; Manakova J; Dihel M; Janca O; Sauer P; Klimkova M; Strakova P; Kvapil P Virol J 2021[May]; 18 (1): 95 PMID33947425show ga The early identification of asymptomatic yet infectious cases is vital to curb the 2019 coronavirus (COVID-19) pandemic and to control the disease in the post-pandemic era. In this paper, we propose a fast, inexpensive and high-throughput approach using painless nasal-swab self-collection followed by direct RT-qPCR for the sensitive PCR detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This approach was validated in a large prospective cohort study of 1038 subjects, analysed simultaneously using (1) nasopharyngeal swabs obtained with the assistance of healthcare personnel and analysed by classic two-step RT-qPCR on RNA isolates and (2) nasal swabs obtained by self-collection and analysed with direct RT-qPCR. Of these subjects, 28.6% tested positive for SARS-CoV-2 using nasopharyngeal swab sampling. Our direct RT-qPCR approach for self-collected nasal swabs performed well with results similar to those of the two-step RT-qPCR on RNA isolates, achieving 0.99 positive and 0.98 negative predictive values (cycle threshold [Ct] < 37). Our research also reports on grey-zone viraemia, including samples with near-cut-off Ct values (Ct >/= 37). In all investigated subjects (n = 20) with grey-zone viraemia, the ultra-small viral load disappeared within hours or days with no symptoms. Overall, this study underscores the importance of painless nasal-swab self-collection and direct RT-qPCR for mass testing during the SARS-CoV-2 pandemic and in the post-pandemic era. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/*prevention & control[MESH] |Clinical Laboratory Techniques/methods[MESH] |Diagnostic Tests, Routine/methods[MESH] |Humans[MESH] |Mass Screening/*methods[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/genetics[MESH] |Self-Examination/*methods[MESH] |Sensitivity and Specificity[MESH] |Specimen Handling/methods[MESH] |Surveys and Questionnaires[MESH]Excellent option for mass testing during the SARS-CoV-2 pandemic: pain(epmc).pdf DeepDyve Pubget Overpricing