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10.1182/blood.2021012064

http://scihub22266oqcxt.onion/10.1182/blood.2021012064
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33945605!8105122!33945605
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suck abstract from ncbi


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pmid33945605      Blood 2021 ; 137 (26): 3656-3659
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  • A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination #MMPMID33945605
  • Handtke S; Wolff M; Zaninetti C; Wesche J; Schonborn L; Aurich K; Ulm L; Hubner NO; Becker K; Thiele T; Greinacher A
  • Blood 2021[Jul]; 137 (26): 3656-3659 PMID33945605show ga
  • Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119.
  • |*SARS-CoV-2[MESH]
  • |Antibody Specificity[MESH]
  • |Autoantibodies/biosynthesis/*blood/immunology[MESH]
  • |COVID-19 Vaccines/*adverse effects/immunology[MESH]
  • |COVID-19/*prevention & control[MESH]
  • |ChAdOx1 nCoV-19[MESH]
  • |Flow Cytometry/*methods[MESH]
  • |Heparin/adverse effects/immunology[MESH]
  • |Humans[MESH]
  • |Immunoenzyme Techniques[MESH]
  • |Immunogenicity, Vaccine[MESH]
  • |Immunoglobulin G/biosynthesis/*blood/immunology[MESH]
  • |P-Selectin/analysis[MESH]
  • |Platelet Activation/*immunology[MESH]
  • |Platelet Factor 4/*immunology[MESH]
  • |Purpura, Thrombocytopenic, Idiopathic/*diagnosis/etiology/immunology[MESH]
  • |Receptors, IgG/*immunology[MESH]


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