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10.1021/jacs.1c02929 http://scihub22266oqcxt.onion/10.1021/jacs.1c02929 33944569!ä!33944569 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Chem+Soc 2021 ; 143 (19): 7261-7266 Nephropedia Template TP {{tp|p=33944569|t=2021. Rapid One-Step Detection of Viral Particles Using an Aptamer-Based Thermophoretic Assay |pdf=|usr=}}{{33944569}} gab.com Text Rapid One-Step Detection of Viral Particles Using an Aptamer-Based Thermophoretic Assay JO: J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=33944569 #FOAvip Rapid and sensitive identification of viral pathogens such as SARS-CoV-2 is a critical step to control the pandemic disease. Viral antigen detection can compete with gold-standard PCR-based nucleic acid diagnostics in terms of better reflection of viral infectivity and reduced risk of contamination from enzymatic amplification. Here, we report the development of a one-step thermophoretic assay using an aptamer and polyethylene glycol (PEG) for direct quantitative detection of viral particles. The assay relies on aptamer binding to the spike protein of SARS-CoV-2 and simultaneous accumulation of aptamer-bound viral particles in laser-induced gradients of temperature and PEG concentration. Using a pseudotyped lentivirus model, a limit of detection of approximately 170 particles muL(-1) (26 fM of the spike protein) is achieved in 15 min without the need of any pretreatment. As a proof of concept, the one-step thermophoretic assay is used to detect synthetic samples by spiking viral particles into oropharyngeal swabs with an accuracy of 100%. The simplicity, speed, and cost-effectiveness of this thermophoretic assay may expand the diagnostic tools for viral pathogens. Twit Text FOAVip Rapid One-Step Detection of Viral Particles Using an Aptamer-Based Thermophoretic Assay ????J Am Chem Soc http://www.kidney.de/pget.php?&tw=1&pmid=33944569 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33944569 #FOAvip English Wikipedia <ref>{{Cite pmid|33944569}}</ref> Rapid One-Step Detection of Viral Particles Using an Aptamer-Based Thermophoretic Assay #MMPMID33944569 Deng J; Tian F; Liu C; Liu Y; Zhao S; Fu T; Sun J; Tan W J Am Chem Soc 2021[May]; 143 (19): 7261-7266 PMID33944569show ga Rapid and sensitive identification of viral pathogens such as SARS-CoV-2 is a critical step to control the pandemic disease. Viral antigen detection can compete with gold-standard PCR-based nucleic acid diagnostics in terms of better reflection of viral infectivity and reduced risk of contamination from enzymatic amplification. Here, we report the development of a one-step thermophoretic assay using an aptamer and polyethylene glycol (PEG) for direct quantitative detection of viral particles. The assay relies on aptamer binding to the spike protein of SARS-CoV-2 and simultaneous accumulation of aptamer-bound viral particles in laser-induced gradients of temperature and PEG concentration. Using a pseudotyped lentivirus model, a limit of detection of approximately 170 particles muL(-1) (26 fM of the spike protein) is achieved in 15 min without the need of any pretreatment. As a proof of concept, the one-step thermophoretic assay is used to detect synthetic samples by spiking viral particles into oropharyngeal swabs with an accuracy of 100%. The simplicity, speed, and cost-effectiveness of this thermophoretic assay may expand the diagnostic tools for viral pathogens. äRapid One-Step Detection of Viral Particles Using an Aptamer-Based The(epmc).pdf DeepDyve Pubget Overpricing