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10.1097/ICO.0000000000002742

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33935238!8244808!33935238
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suck abstract from ncbi


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pmid33935238      Cornea 2021 ; 40 (8): 1044-1047
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  • Role of Reverse Transcriptase Polymerase Chain Reaction in Cornea Donors During the COVID-19 Pandemic #MMPMID33935238
  • Arora R; Sharda S; Sahu J; Manchanda V; Jain P; Gupta I; Gotmare N; Bharti N
  • Cornea 2021[Aug]; 40 (8): 1044-1047 PMID33935238show ga
  • PURPOSE: The purpose of this study was to report the analysis of reverse transcriptase polymerase chain reaction (RT-PCR) of nasopharyngeal (NP) samples of cornea donors dying because of causes unrelated to severe acute respiratory coronavirus (SARS-CoV-2). METHODS: A retrospective analysis of all cornea donors dying from causes other than SARS-CoV-2 between August 2020 and December 2020 was performed. Informed consent was obtained from the next of kin of the deceased for RT-PCR testing from NP swabs. Rapid antigen testing from all the deceased was performed before in situ cornea excision. In addition, NP samples in viral transport media for RT-PCR were also collected for SARS-CoV-2 analysis. Corneas were released from the eye bank only after a negative RT-PCR report. RESULT: One hundred eighteen corneas from 59 donors were obtained by the eye bank. Eleven donors (18.64%) were positive for SARS-CoV-2 on RT-PCR testing. Six of these 11 donors had a Ct value of E gene less than 25. CONCLUSIONS: NP samples of cornea donors dying due to causes other than coronavirus disease-19 were positive for SARS-CoV-2 on RT-PCR. This implicates that donors could be having asymptomatic/undetected coronavirus disease infection. We recommend adding the routine testing of NP samples of all cornea donors in the eye banking protocol in this ongoing SARS-CoV-2 pandemic.
  • |*COVID-19 Nucleic Acid Testing[MESH]
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |Asymptomatic Infections/*mortality[MESH]
  • |COVID-19/*epidemiology[MESH]
  • |Cornea[MESH]
  • |Eye Banks/*statistics & numerical data[MESH]
  • |Humans[MESH]
  • |Middle Aged[MESH]
  • |Nasopharynx/*virology[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Retrospective Studies[MESH]
  • |SARS-CoV-2/*genetics[MESH]
  • |Tissue Donors/*supply & distribution[MESH]


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