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10.1016/j.jcv.2021.104818

http://scihub22266oqcxt.onion/10.1016/j.jcv.2021.104818
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33932848!8015349!33932848
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suck abstract from ncbi


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pmid33932848      J+Clin+Virol 2021 ; 139 (ä): 104818
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  • Evaluation of SARS-CoV-2 total antibody detection via a lateral flow nanoparticle fluorescence immunoassay #MMPMID33932848
  • Sibai M; Solis D; Roltgen K; Stevens BA; Mfuh KO; Sahoo MK; Shi RZ; Zehnder J; Boyd SD; Pinsky BA
  • J Clin Virol 2021[Jun]; 139 (ä): 104818 PMID33932848show ga
  • BACKGROUND: The coronavirus disease 2019 (COVID-19) endgame may benefit from simple, accurate antibody testing to characterize seroprevalence and immunization coverage. OBJECTIVES: To evaluate the performance of the lateral flow QIAreach anti-SARS-CoV-2 Total rapid nanoparticle fluorescence immunoassay compared to reference isotype-specific IgG, IgM, and IgA SARS-CoV-2 ELISA using S1 or receptor binding domain (RBD) as antigens. STUDY DESIGN: A diagnostic comparison study was carried out using 154 well-characterized heparin plasma samples. Agreement between assays was assessed by overall, positive, and negative percent agreement and Cohen's kappa coefficient. RESULTS: Overall agreement between the QIAreach anti-SARS-CoV-2 Total and any anti-spike domain (S1 or RBD) antibody isotype was 96.0 % (95 % CI 89.8-98.8), the positive percent agreement was 97.6 % (95 % CI 91.0-99.9), the negative percent agreement was 88.2 % (95 % CI 64.4-98.0). The kappa coefficient was 0.86 (95 % CI 0.72 to 0.99). CONCLUSION: The QIAreach anti-SARS-CoV-2 Total rapid antibody test provides comparable performance to high-complexity, laboratory-based ELISA.
  • |Adult[MESH]
  • |Aged[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Enzyme-Linked Immunosorbent Assay[MESH]
  • |Female[MESH]
  • |Fluorescent Antibody Technique/*methods[MESH]
  • |Humans[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Nanoparticles[MESH]


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