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10.1016/j.prp.2021.153443 http://scihub22266oqcxt.onion/10.1016/j.prp.2021.153443 33930607!8045416!33930607     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pathol+Res+Pract 2021 ; 221 (ä): 153443 Nephropedia Template TP {{tp|p=33930607|t=2021. Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19 |pdf=|usr=}}{{33930607}} gab.com Text Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19 JO: Pathol Res Pract http://www.kidney.de/pget.php?&tw=1&pmid=33930607 #FOAvip Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the control of virus spread has remained challenging given the pitfalls of the current diagnostic tests. Nevertheless, RNA amplification techniques have been the gold standard among other diagnostic methods for monitoring clinical samples for the presence of the virus. In the current paper, we review the shortcomings and strengths of RT-PCR (real-time polymerase chain reaction) techniques for diagnosis of coronavirus disease (COVID)-19. We address the repercussions of false-negative and false-positive rates encountered in the test, summarize approaches to improve the overall sensitivity of this method. We discuss the barriers to the widespread use of the RT-PCR test, and some technical advances, such as RT-LAMP (reverse-transcriptase-loop mediated isothermal amplification). We also address how other molecular techniques, such as immunodiagnostic tests can be used to avoid incorrect interpretation of RT-PCR tests. Twit Text FOAVip Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19 ????Pathol Res Pract http://www.kidney.de/pget.php?&tw=1&pmid=33930607 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33930607 #FOAvip English Wikipedia <ref>{{Cite pmid|33930607}}</ref> Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19 #MMPMID33930607 Teymouri M; Mollazadeh S; Mortazavi H; Naderi Ghale-Noie Z; Keyvani V; Aghababaei F; Hamblin MR; Abbaszadeh-Goudarzi G; Pourghadamyari H; Hashemian SMR; Mirzaei H Pathol Res Pract 2021[May]; 221 (ä): 153443 PMID33930607show ga Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the control of virus spread has remained challenging given the pitfalls of the current diagnostic tests. Nevertheless, RNA amplification techniques have been the gold standard among other diagnostic methods for monitoring clinical samples for the presence of the virus. In the current paper, we review the shortcomings and strengths of RT-PCR (real-time polymerase chain reaction) techniques for diagnosis of coronavirus disease (COVID)-19. We address the repercussions of false-negative and false-positive rates encountered in the test, summarize approaches to improve the overall sensitivity of this method. We discuss the barriers to the widespread use of the RT-PCR test, and some technical advances, such as RT-LAMP (reverse-transcriptase-loop mediated isothermal amplification). We also address how other molecular techniques, such as immunodiagnostic tests can be used to avoid incorrect interpretation of RT-PCR tests. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH]Recent advances and challenges of RT-PCR tests for the diagnosis of CO(epmc).pdf DeepDyve Pubget Overpricing