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10.3390/v13050786 http://scihub22266oqcxt.onion/10.3390/v13050786 33925055!8146997!33925055     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2021 ; 13 (5): ä Nephropedia Template TP {{tp|p=33925055|t=2021. Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort |pdf=|usr=}}{{33925055}} gab.com Text Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33925055 #FOAvip The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100% sensitivity and specificity) in a case-control cohort (n = 100). We then assessed the influence of disease severity, age, and ethnicity on the strength and breadth of the humoral response in a multi-ethnic cohort (n = 138). As expected, patients with severe disease showed significantly higher antibody titers and interestingly also had significantly broader epitope coverage. A significant increase in antibody titer and epitope coverage was observed with increasing age, in both mild and severe disease, which is promising for vaccine efficacy in older individuals. Additionally, we observed significant differences in the breadth and strength of the humoral immune response in relation to ethnicity, which may reflect differences in genetic and lifestyle factors. Furthermore, our data enabled localization of the immuno-dominant epitope to the C-terminal structural domain of the viral nucleocapsid protein in two independent cohorts. Overall, we have designed, validated, and tested an advanced serological assay that enables accurate quantitation of the humoral response post natural infection and that has revealed unexpected differences in the magnitude and epitope utilization within a population. Twit Text FOAVip Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33925055 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33925055 #FOAvip English Wikipedia <ref>{{Cite pmid|33925055}}</ref> Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort #MMPMID33925055 Smith M; Abdesselem HB; Mullins M; Tan TM; Nel AJM; Al-Nesf MAY; Bensmail I; Majbour NK; Vaikath NN; Naik A; Ouararhni K; Mohamed-Ali V; Al-Maadheed M; Schell DT; Baros-Steyl SS; Anuar ND; Ismail NH; Morris PE; Mamat RNR; Rosli NSM; Anwar A; Ellan K; Zain RM; Burgers WA; Mayne ES; El-Agnaf OMA; Blackburn JM Viruses 2021[Apr]; 13 (5): ä PMID33925055show ga The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100% sensitivity and specificity) in a case-control cohort (n = 100). We then assessed the influence of disease severity, age, and ethnicity on the strength and breadth of the humoral response in a multi-ethnic cohort (n = 138). As expected, patients with severe disease showed significantly higher antibody titers and interestingly also had significantly broader epitope coverage. A significant increase in antibody titer and epitope coverage was observed with increasing age, in both mild and severe disease, which is promising for vaccine efficacy in older individuals. Additionally, we observed significant differences in the breadth and strength of the humoral immune response in relation to ethnicity, which may reflect differences in genetic and lifestyle factors. Furthermore, our data enabled localization of the immuno-dominant epitope to the C-terminal structural domain of the viral nucleocapsid protein in two independent cohorts. Overall, we have designed, validated, and tested an advanced serological assay that enables accurate quantitation of the humoral response post natural infection and that has revealed unexpected differences in the magnitude and epitope utilization within a population. |Adolescent[MESH] |Adult[MESH] |Antibodies, Viral/*immunology[MESH] |Antigens, Viral/immunology[MESH] |COVID-19 Serological Testing[MESH] |COVID-19/epidemiology/*immunology/virology[MESH] |Case-Control Studies[MESH] |Cohort Studies[MESH] |Epitopes[MESH] |Ethnicity[MESH] |Female[MESH] |Humans[MESH] |Immunity, Humoral[MESH] |Immunoglobulin G/immunology[MESH] |Male[MESH] |Middle Aged[MESH] |Nucleocapsid Proteins/genetics/immunology[MESH] |Pandemics[MESH] |SARS-CoV-2/genetics/*immunology[MESH] |Sensitivity and Specificity[MESH] |Seroepidemiologic Studies[MESH] |Severity of Illness Index[MESH]Age, Disease Severity and Ethnicity Influence Humoral Responses in a M(epmc).pdf DeepDyve Pubget Overpricing