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10.3390/vaccines9040373 http://scihub22266oqcxt.onion/10.3390/vaccines9040373 33921215!8068889!33921215     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Vaccines+(Basel) 2021 ; 9 (4): ä Nephropedia Template TP {{tp|p=33921215|t=2021. The C-Terminal Domain of Nef(mut) Is Dispensable for the CD8(+) T Cell Immunogenicity of In Vivo Engineered Extracellular Vesicles |pdf=|usr=}}{{33921215}} gab.com Text The C-Terminal Domain of Nef(mut) Is Dispensable for the CD8(+) T Cell Immunogenicity of In Vivo Engineered Extracellular Vesicles JO: Vaccines (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33921215 #FOAvip Intramuscular injection of DNA vectors expressing the extracellular vesicle (EV)-anchoring protein Nef(mut) fused at its C-terminus to viral and tumor antigens elicit a potent, effective, and anti-tolerogenic CD8(+) T cell immunity against the heterologous antigen. The immune response is induced through the production of EVs incorporating Nef(mut)-derivatives released by muscle cells. In the perspective of a possible translation into the clinic of the Nef(mut)-based vaccine platform, we aimed at increasing its safety profile by identifying the minimal part of Nef(mut) retaining the EV-anchoring protein property. We found that a C-terminal deletion of 29-amino acids did not affect the ability of Nef(mut) to associate with EVs. The EV-anchoring function was also preserved when antigens from both HPV16 (i.e., E6 and E7) and SARS-CoV-2 (i.e., S1 and S2) were fused to its C-terminus. Most important, the Nef(mut) C-terminal deletion did not affect levels, quality, and diffusion at distal sites of the antigen-specific CD8(+) T immunity. We concluded that the C-terminal Nef(mut) truncation does not influence stability, EV-anchoring, and CD8(+) T cell immunogenicity of the fused antigen. Hence, the C-terminal deleted Nef(mut) may represent a safer alternative to the full-length isoform for vaccines in humans. Twit Text FOAVip The C-Terminal Domain of Nef(mut) Is Dispensable for the CD8(+) T Cell Immunogenicity of In Vivo Engineered Extracellular Vesicles ????Vaccines (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33921215 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33921215 #FOAvip English Wikipedia <ref>{{Cite pmid|33921215}}</ref> The C-Terminal Domain of Nef(mut) Is Dispensable for the CD8(+) T Cell Immunogenicity of In Vivo Engineered Extracellular Vesicles #MMPMID33921215 Chiozzini C; Manfredi F; Ferrantelli F; Leone P; Giovannelli A; Olivetta E; Federico M Vaccines (Basel) 2021[Apr]; 9 (4): ä PMID33921215show ga Intramuscular injection of DNA vectors expressing the extracellular vesicle (EV)-anchoring protein Nef(mut) fused at its C-terminus to viral and tumor antigens elicit a potent, effective, and anti-tolerogenic CD8(+) T cell immunity against the heterologous antigen. The immune response is induced through the production of EVs incorporating Nef(mut)-derivatives released by muscle cells. In the perspective of a possible translation into the clinic of the Nef(mut)-based vaccine platform, we aimed at increasing its safety profile by identifying the minimal part of Nef(mut) retaining the EV-anchoring protein property. We found that a C-terminal deletion of 29-amino acids did not affect the ability of Nef(mut) to associate with EVs. The EV-anchoring function was also preserved when antigens from both HPV16 (i.e., E6 and E7) and SARS-CoV-2 (i.e., S1 and S2) were fused to its C-terminus. Most important, the Nef(mut) C-terminal deletion did not affect levels, quality, and diffusion at distal sites of the antigen-specific CD8(+) T immunity. We concluded that the C-terminal Nef(mut) truncation does not influence stability, EV-anchoring, and CD8(+) T cell immunogenicity of the fused antigen. Hence, the C-terminal deleted Nef(mut) may represent a safer alternative to the full-length isoform for vaccines in humans. äThe C-Terminal Domain of Nef(mut) Is Dispensable for the CD8(+) T Cell(epmc).pdf DeepDyve Pubget Overpricing