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10.3390/v13040655 http://scihub22266oqcxt.onion/10.3390/v13040655 33920222!8069482!33920222     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2021 ; 13 (4): ä Nephropedia Template TP {{tp|p=33920222|t=2021. SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus |pdf=|usr=}}{{33920222}} gab.com Text SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33920222 #FOAvip SARS-CoV-2 serum neutralization assay represents the gold standard for assessing antibody-mediated protection in naturally infected and vaccinated individuals. In the present study, 662 serum samples collected from February 2020 to January 2021 from acute and convalescent COVID-19 patients were tested to determine neutralizing antibody (NAb) titers using a microneutralization test (MNT) for live SARS-CoV-2. Moreover, anti-SARS-CoV-2 IgG, IgA, and IgM directed against different viral antigens were measured by high-throughput automated platforms. We observed higher levels of NAbs in elderly (>60 years old) individuals and in patients presenting acute respiratory distress syndrome. SARS-CoV-2 NAbs develop as soon as five days from symptom onset and, despite a decline after the second month, persist for over 11 months, showing variable dynamics. Through correlation and receiver operating characteristic (ROC) curve analysis, we set up a testing algorithm, suitable for the laboratory workload, by establishing an optimal cutoff value of anti-SARS-CoV-2 IgG for convalescent plasma donors to exclude from MNT samples foreseen to have low/negative NAb titers and ineligible for plasma donation. Overall, MNT, although cumbersome and not suitable for routine testing of large sample sizes, remains the reference tool for the assessment of antibody-mediated immunity after SARS-CoV-2 infection. Smart testing algorithms may optimize the laboratory workflow to monitor antibody-mediated protection in COVID-19 patients, plasma donors, and vaccinated individuals. Twit Text FOAVip SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33920222 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33920222 #FOAvip English Wikipedia <ref>{{Cite pmid|33920222}}</ref> SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-New Virus #MMPMID33920222 Matusali G; Colavita F; Lapa D; Meschi S; Bordi L; Piselli P; Gagliardini R; Corpolongo A; Nicastri E; Antinori A; Ippolito G; Capobianchi MR; Castilletti C; Inmi Covid-Laboratory Team Viruses 2021[Apr]; 13 (4): ä PMID33920222show ga SARS-CoV-2 serum neutralization assay represents the gold standard for assessing antibody-mediated protection in naturally infected and vaccinated individuals. In the present study, 662 serum samples collected from February 2020 to January 2021 from acute and convalescent COVID-19 patients were tested to determine neutralizing antibody (NAb) titers using a microneutralization test (MNT) for live SARS-CoV-2. Moreover, anti-SARS-CoV-2 IgG, IgA, and IgM directed against different viral antigens were measured by high-throughput automated platforms. We observed higher levels of NAbs in elderly (>60 years old) individuals and in patients presenting acute respiratory distress syndrome. SARS-CoV-2 NAbs develop as soon as five days from symptom onset and, despite a decline after the second month, persist for over 11 months, showing variable dynamics. Through correlation and receiver operating characteristic (ROC) curve analysis, we set up a testing algorithm, suitable for the laboratory workload, by establishing an optimal cutoff value of anti-SARS-CoV-2 IgG for convalescent plasma donors to exclude from MNT samples foreseen to have low/negative NAb titers and ineligible for plasma donation. Overall, MNT, although cumbersome and not suitable for routine testing of large sample sizes, remains the reference tool for the assessment of antibody-mediated immunity after SARS-CoV-2 infection. Smart testing algorithms may optimize the laboratory workflow to monitor antibody-mediated protection in COVID-19 patients, plasma donors, and vaccinated individuals. |Adult[MESH] |Algorithms[MESH] |Antibodies, Neutralizing/blood[MESH] |Antibodies, Viral/blood[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/blood/diagnosis/*immunology[MESH] |Female[MESH] |Humans[MESH] |Immunoglobulin G/blood[MESH] |Kinetics[MESH] |Male[MESH] |Middle Aged[MESH] |Neutralization Tests/*methods[MESH] |ROC Curve[MESH]SARS-CoV-2 Serum Neutralization Assay: A Traditional Tool for a Brand-(epmc).pdf DeepDyve Pubget Overpricing