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10.3390/v13040599 http://scihub22266oqcxt.onion/10.3390/v13040599 33915875!8067199!33915875     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2021 ; 13 (4): ä Nephropedia Template TP {{tp|p=33915875|t=2021. Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera |pdf=|usr=}}{{33915875}} gab.com Text Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33915875 #FOAvip Coronavirus (CoV) spillover events from wildlife reservoirs can result in mild to severe human respiratory illness. These spillover events underlie the importance of detecting known and novel CoVs circulating in reservoir host species and determining CoV prevalence and distribution, allowing improved prediction of spillover events or where a human-reservoir interface should be closely monitored. To increase the likelihood of detecting all circulating genera and strains, we have modified primers published by Watanabe et al. in 2010 to generate a semi-nested pan-CoV PCR assay. Representatives from the four coronavirus genera (alpha-CoVs, beta-CoVs, gamma-CoVs and delta-CoVs) were tested and all of the in-house CoVs were detected using this assay. After comparing both assays, we found that the updated assay reliably detected viruses in all genera of CoVs with high sensitivity, whereas the sensitivity of the original assay was lower. Our updated PCR assay is an important tool to detect, monitor and track CoVs to enhance viral surveillance in reservoir hosts. Twit Text FOAVip Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33915875 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33915875 #FOAvip English Wikipedia <ref>{{Cite pmid|33915875}}</ref> Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera #MMPMID33915875 Holbrook MG; Anthony SJ; Navarrete-Macias I; Bestebroer T; Munster VJ; van Doremalen N Viruses 2021[Apr]; 13 (4): ä PMID33915875show ga Coronavirus (CoV) spillover events from wildlife reservoirs can result in mild to severe human respiratory illness. These spillover events underlie the importance of detecting known and novel CoVs circulating in reservoir host species and determining CoV prevalence and distribution, allowing improved prediction of spillover events or where a human-reservoir interface should be closely monitored. To increase the likelihood of detecting all circulating genera and strains, we have modified primers published by Watanabe et al. in 2010 to generate a semi-nested pan-CoV PCR assay. Representatives from the four coronavirus genera (alpha-CoVs, beta-CoVs, gamma-CoVs and delta-CoVs) were tested and all of the in-house CoVs were detected using this assay. After comparing both assays, we found that the updated assay reliably detected viruses in all genera of CoVs with high sensitivity, whereas the sensitivity of the original assay was lower. Our updated PCR assay is an important tool to detect, monitor and track CoVs to enhance viral surveillance in reservoir hosts. |Animals[MESH] |Animals, Wild[MESH] |Clinical Laboratory Techniques/methods[MESH] |Coronavirus Infections/virology[MESH] |Coronavirus/*classification/*genetics/*isolation & purification[MESH] |Disease Reservoirs/virology[MESH] |Genome, Viral[MESH] |Host Specificity[MESH] |Humans[MESH] |Limit of Detection[MESH] |Pandemics[MESH] |Phylogeny[MESH] |Polymerase Chain Reaction/*methods[MESH]Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavi(epmc).pdf DeepDyve Pubget Overpricing