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10.1016/j.bios.2021.113177 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113177 33915435!7987504!33915435     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 185 (ä): 113177 Nephropedia Template TP {{tp|p=33915435|t=2021. Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 (COVID-19) |pdf=|usr=}}{{33915435}} gab.com Text Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 (COVID-19) JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33915435 #FOAvip Rapid diagnosis and case isolation are pivotal to controlling the current pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, a label-free DNA capacitive biosensor for the detection of SARS-CoV-2 that demonstrates real-time, low-cost, and high-throughput screening of nucleic acid samples is presented. Our novel biosensor composed of the interdigitated platinum/titanium electrodes on the glass substrate can detect the hybridization of analyte DNA with probe DNA. The hybridization signals of specific DNA sequences were verified through exhaustive physicochemical analytical techniques such as Fourier transform infrared (FT-IR) spectrometry, contact-angle analysis, and capacitance-frequency measurements. For a single-step hybridized reaction, the fabricated kit exhibited significant sensitivity (capacitance change, DeltaC = ~2 nF) in detecting the conserved region of the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) gene with high sensitivity of 0.843 nF/nM. In addition to capacitive measurements, this selective detection was confirmed by the fluorescence image and intensity from a SARS-CoV-2 gene labeled with a fluorescent dye. We also demonstrated that the kits are recyclable by surface ozone treatment using UV irradiation. Thus, these kits could potentially be applied to various types of label-free DNA, thereby acting as rapid, cost-effective biosensors for several diseases. Twit Text FOAVip Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 (COVID-19) ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33915435 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33915435 #FOAvip English Wikipedia <ref>{{Cite pmid|33915435}}</ref> Ultra-fast and recyclable DNA biosensor for point-of-care detection of SARS-CoV-2 (COVID-19) #MMPMID33915435 Hwang C; Park N; Kim ES; Kim M; Kim SD; Park S; Kim NY; Kim JH Biosens Bioelectron 2021[Aug]; 185 (ä): 113177 PMID33915435show ga Rapid diagnosis and case isolation are pivotal to controlling the current pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, a label-free DNA capacitive biosensor for the detection of SARS-CoV-2 that demonstrates real-time, low-cost, and high-throughput screening of nucleic acid samples is presented. Our novel biosensor composed of the interdigitated platinum/titanium electrodes on the glass substrate can detect the hybridization of analyte DNA with probe DNA. The hybridization signals of specific DNA sequences were verified through exhaustive physicochemical analytical techniques such as Fourier transform infrared (FT-IR) spectrometry, contact-angle analysis, and capacitance-frequency measurements. For a single-step hybridized reaction, the fabricated kit exhibited significant sensitivity (capacitance change, DeltaC = ~2 nF) in detecting the conserved region of the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) gene with high sensitivity of 0.843 nF/nM. In addition to capacitive measurements, this selective detection was confirmed by the fluorescence image and intensity from a SARS-CoV-2 gene labeled with a fluorescent dye. We also demonstrated that the kits are recyclable by surface ozone treatment using UV irradiation. Thus, these kits could potentially be applied to various types of label-free DNA, thereby acting as rapid, cost-effective biosensors for several diseases. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |DNA[MESH] |Humans[MESH] |Point-of-Care Systems[MESH] |RNA, Viral[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH]Ultra-fast and recyclable DNA biosensor for point-of-care detection of(epmc).pdf DeepDyve Pubget Overpricing