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10.1371/journal.pone.0250942 http://scihub22266oqcxt.onion/10.1371/journal.pone.0250942 33914804!8084238!33914804     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33914804&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (4): e0250942 Nephropedia Template TP {{tp|p=33914804|t=2021. Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2 |pdf=|usr=}}{{33914804}} gab.com Text Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2 JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33914804 #FOAvip The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV) continues to increase globally. The real-time reverse transcription polymerase chain reaction (rRT-PCR) is the most used technique in virus detection. However, possible false-negative and false-positive results produce misleading consequences, making it necessary to improve existing methods. Here, we developed a multiplex rRT-PCR diagnostic method, which targets two viral genes (RdRP and E) and one human gene (RP) simultaneously. The reaction was tested by using pseudoviral RNA and human target mRNA sequences as a template. Also, the protocol was validated by using 14 clinical SARS-CoV-2 positive samples. The results are in good agreement with the CDC authorized Cepheid;s Xpert(R) Xpress SARS-CoV-2 diagnostic system (100%). Unlike single gene targeting strategies, the current method provides the amplification of two viral regions in the same PCR reaction. Therefore, an accurate SARS-CoV-2 diagnostic assay was provided, which allows testing of 91 samples in 96-well plates in per run. Thanks to this strategy, fast, reliable, and easy-to-use rRT-PCR method is obtained to diagnose SARS-CoV-2. Twit Text FOAVip Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2 ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33914804 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33914804 #FOAvip English Wikipedia <ref>{{Cite pmid|33914804}}</ref> Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2 #MMPMID33914804 Tombuloglu H; Sabit H; Al-Suhaimi E; Al Jindan R; Alkharsah KR PLoS One 2021[]; 16 (4): e0250942 PMID33914804show ga The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV) continues to increase globally. The real-time reverse transcription polymerase chain reaction (rRT-PCR) is the most used technique in virus detection. However, possible false-negative and false-positive results produce misleading consequences, making it necessary to improve existing methods. Here, we developed a multiplex rRT-PCR diagnostic method, which targets two viral genes (RdRP and E) and one human gene (RP) simultaneously. The reaction was tested by using pseudoviral RNA and human target mRNA sequences as a template. Also, the protocol was validated by using 14 clinical SARS-CoV-2 positive samples. The results are in good agreement with the CDC authorized Cepheid;s Xpert(R) Xpress SARS-CoV-2 diagnostic system (100%). Unlike single gene targeting strategies, the current method provides the amplification of two viral regions in the same PCR reaction. Therefore, an accurate SARS-CoV-2 diagnostic assay was provided, which allows testing of 91 samples in 96-well plates in per run. Thanks to this strategy, fast, reliable, and easy-to-use rRT-PCR method is obtained to diagnose SARS-CoV-2. |COVID-19 Nucleic Acid Testing/*methods/standards[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Limit of Detection[MESH] |Multiplex Polymerase Chain Reaction/*methods/standards[MESH] |RNA, Viral/analysis/*genetics[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]Development of multiplex real-time RT-PCR assay for the detection of S(epmc).pdf DeepDyve Pubget Overpricing