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10.1038/s41598-021-88315-3 http://scihub22266oqcxt.onion/10.1038/s41598-021-88315-3 33911109!8080620!33911109     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 9136 Nephropedia Template TP {{tp|p=33911109|t=2021. Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion |pdf=|usr=}}{{33911109}} gab.com Text Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33911109 #FOAvip Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the design and versatile applications of orthogonal CC dimer-forming peptides with a dissociation constant in the low nanomolar range. In vitro stability and specificity was confirmed in mammalian cells by enzyme reconstitution, transcriptional activation using a combination of DNA-binding and a transcriptional activation domain, and cellular-enzyme-activity regulation based on externally-added peptides. In addition to cellular regulation, coiled-coil-mediated reporter reconstitution was used for the detection of cell fusion mediated by the interaction between the spike protein of pandemic SARS-CoV2 and the ACE2 receptor. This assay can be used to investigate the mechanism of viral spike protein-mediated fusion or screening for viral inhibitors under biosafety level 1 conditions. Twit Text FOAVip Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33911109 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33911109 #FOAvip English Wikipedia <ref>{{Cite pmid|33911109}}</ref> Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion #MMPMID33911109 Plaper T; Aupic J; Dekleva P; Lapenta F; Keber MM; Jerala R; Bencina M Sci Rep 2021[Apr]; 11 (1): 9136 PMID33911109show ga Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay. Here, we report the design and versatile applications of orthogonal CC dimer-forming peptides with a dissociation constant in the low nanomolar range. In vitro stability and specificity was confirmed in mammalian cells by enzyme reconstitution, transcriptional activation using a combination of DNA-binding and a transcriptional activation domain, and cellular-enzyme-activity regulation based on externally-added peptides. In addition to cellular regulation, coiled-coil-mediated reporter reconstitution was used for the detection of cell fusion mediated by the interaction between the spike protein of pandemic SARS-CoV2 and the ACE2 receptor. This assay can be used to investigate the mechanism of viral spike protein-mediated fusion or screening for viral inhibitors under biosafety level 1 conditions. |Angiotensin-Converting Enzyme 2/genetics/metabolism[MESH] |Cell Fusion[MESH] |Circular Dichroism[MESH] |Giant Cells/virology[MESH] |Green Fluorescent Proteins/genetics/metabolism[MESH] |HEK293 Cells[MESH] |Host-Pathogen Interactions/*physiology[MESH] |Humans[MESH] |Luciferases/genetics/metabolism[MESH] |Membrane Fusion[MESH] |Peptides/*chemistry/genetics/*metabolism[MESH] |Protein Engineering/methods[MESH] |Protein Multimerization[MESH] |Protein Stability[MESH] |Serine Endopeptidases/genetics/metabolism[MESH] |Spike Glycoprotein, Coronavirus/*metabolism[MESH]Coiled-coil heterodimers with increased stability for cellular regulat(epmc).pdf DeepDyve Pubget Overpricing