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10.1126/science.abe7106 http://scihub22266oqcxt.onion/10.1126/science.abe7106 33906967!8224270!33906967     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2021 ; 372 (6545): 941-948 Nephropedia Template TP {{tp|p=33906967|t=2021. Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9 |pdf=|usr=}}{{33906967}} gab.com Text Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9 JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=33906967 #FOAvip CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their processing and utilization by Cas9. While analyzing Cas9-RNA complexes from Campylobacter jejuni, we discovered tracrRNA hybridizing to cellular RNAs, leading to formation of "noncanonical" crRNAs capable of guiding DNA targeting by Cas9. Our discovery inspired the engineering of reprogrammed tracrRNAs that link the presence of any RNA of interest to DNA targeting with different Cas9 orthologs. This capability became the basis for a multiplexable diagnostic platform termed LEOPARD (leveraging engineered tracrRNAs and on-target DNAs for parallel RNA detection). LEOPARD allowed simultaneous detection of RNAs from different viruses in one test and distinguished severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its D614G (Asp(614)-->Gly) variant with single-base resolution in patient samples. Twit Text FOAVip Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9 ????Science http://www.kidney.de/pget.php?&tw=1&pmid=33906967 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33906967 #FOAvip English Wikipedia <ref>{{Cite pmid|33906967}}</ref> Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9 #MMPMID33906967 Jiao C; Sharma S; Dugar G; Peeck NL; Bischler T; Wimmer F; Yu Y; Barquist L; Schoen C; Kurzai O; Sharma CM; Beisel CL Science 2021[May]; 372 (6545): 941-948 PMID33906967show ga CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their processing and utilization by Cas9. While analyzing Cas9-RNA complexes from Campylobacter jejuni, we discovered tracrRNA hybridizing to cellular RNAs, leading to formation of "noncanonical" crRNAs capable of guiding DNA targeting by Cas9. Our discovery inspired the engineering of reprogrammed tracrRNAs that link the presence of any RNA of interest to DNA targeting with different Cas9 orthologs. This capability became the basis for a multiplexable diagnostic platform termed LEOPARD (leveraging engineered tracrRNAs and on-target DNAs for parallel RNA detection). LEOPARD allowed simultaneous detection of RNAs from different viruses in one test and distinguished severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its D614G (Asp(614)-->Gly) variant with single-base resolution in patient samples. |*Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Base Sequence[MESH] |COVID-19 Nucleic Acid Testing[MESH] |COVID-19/diagnosis/virology[MESH] |CRISPR-Associated Protein 9/*metabolism[MESH] |CRISPR-Cas Systems[MESH] |Campylobacter jejuni[MESH] |Humans[MESH] |Nucleic Acid Hybridization[MESH] |RNA, Bacterial/genetics[MESH] |RNA, Guide, CRISPR-Cas Systems/*genetics[MESH] |RNA, Messenger/genetics[MESH] |RNA, Viral/*analysis/genetics[MESH] |RNA/*analysis/*genetics[MESH] |SARS-CoV-2/*genetics[MESH]Noncanonical crRNAs derived from host transcripts enable multiplexable(epmc).pdf DeepDyve Pubget Overpricing