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10.1016/j.ijid.2021.04.066

http://scihub22266oqcxt.onion/10.1016/j.ijid.2021.04.066
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suck abstract from ncbi


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pmid33901651      Int+J+Infect+Dis 2021 ; 107 (ä): 179-181
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  • Performance evaluation of the QIAstat-Dx(R) Respiratory SARS-CoV-2 Panel #MMPMID33901651
  • Lebourgeois S; Storto A; Gout B; Le Hingrat Q; Ardila Tjader G; Cerdan MDC; English A; Pareja J; Love J; Houhou-Fidouh N; Manissero D; Descamps D; Visseaux B
  • Int J Infect Dis 2021[Jun]; 107 (ä): 179-181 PMID33901651show ga
  • OBJECTIVE: The aim of this study was to evaluate the QIAstat-Dx(R) Respiratory SARS-CoV-2 Panel (QIAstat-SARS-CoV-2), which is a closed, fully automated, multiplex polymerase chain reaction (PCR) assay that detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and 21 other pathogens that cause respiratory disease. METHODS: Nasopharyngeal swabs from patients with or suspected of having coronavirus disease 2019 were collected and tested at Bichat-Claude Bernard Hospital, Paris, France. Using the World Health Organisation-approved real-time-PCR assay developed by the Charite Institute of Virology as the reference, positive percent agreement (PPA) and negative percent agreement (NPA) were calculated. RESULTS: In total, 189 negative and 88 positive samples were analyzed. QIAstat-SARS-CoV-2 had an NPA of 90.48% (95% confidence interval (CI), 85.37%, 94.26%) and a PPA of 94.32% (95% CI, 87.24%, 98.13%). Co-infections were detected by QIAstat-SARS-CoV-2 in 4/277 specimens. The methods exhibited comparable failure rates (23/307 [7.5%] vs. 6/298 [2.0%] for QIAstat-SARS-CoV-2 and reference methods, respectively). The turnaround time was shorter for QIAstat-SARS-CoV-2 compared with the reference method (difference in mean -14:30 h [standard error, 0:03:23; 95% CI, -14:37, -14:24]; P < 0.001). CONCLUSIONS: QIAstat-SARS-CoV-2 shows good agreement with the reference assay, providing faster and accurate results for detecting SARS-CoV-2.
  • |Adolescent[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Aged, 80 and over[MESH]
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Child[MESH]
  • |Child, Preschool[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Infant[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Multiplex Polymerase Chain Reaction/*methods[MESH]
  • |Nasopharynx/virology[MESH]
  • |Retrospective Studies[MESH]
  • |SARS-CoV-2/*genetics[MESH]


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