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Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 ACS+Appl+Mater+Interfaces 2021 ; 13 (17): 19816-19824 Nephropedia Template TP
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Rational Engineering of the DNA Walker Amplification Strategy by Using a Au@Ti(3)C(2)@PEI-Ru(dcbpy)(3)(2+) Nanocomposite Biosensor for Detection of the SARS-CoV-2 RdRp Gene #MMPMID33890471
Yao B; Zhang J; Fan Z; Ding Y; Zhou B; Yang R; Zhao J; Zhang K
ACS Appl Mater Interfaces 2021[May]; 13 (17): 19816-19824 PMID33890471show ga
The detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for preventing and controlling infectious diseases and disease treatment. In this work, a Au@Ti(3)C(2)@PEI-Ru(dcbpy)(3)(2+) nanocomposite-based electrochemiluminescence (ECL) biosensor was rationally designed, which realized sensitive detection of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. In addition, a DNA walker was also used to excise the hairpin DNAs under the action of Nb.BbvCI endonuclease. Furthermore, model DNA-Ag nanoclusters (model DNA-AgNCs) were used to quench the initial ECL signal. As a result, the ECL biosensor was used to sensitively detect the SARS-CoV-2 RdRp gene with a detection range of 1 fM to 100 pM and a limit of detection of 0.21 fM. It was indicated that the ECL biosensor had a great application potential for clinical medical detection. Furthermore, the DNA walker amplification also played a reliable candidate strategy for other detection methods.