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10.1016/j.ymeth.2021.04.011 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2021.04.011 33882362!8105137!33882362     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods 2022 ; 201 (ä): 15-25 Nephropedia Template TP {{tp|p=33882362|t=2022. Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts |pdf=|usr=}}{{33882362}} gab.com Text Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=33882362 #FOAvip The replication of SARS-CoV-2 and other coronaviruses depends on transcription of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous transcription. Recent studies have aimed to characterize the expression of subgenomic SARS-CoV-2 transcripts in order to investigate their clinical significance. Here, we describe a novel panel of reverse transcription droplet digital PCR (RT-ddPCR) assays designed to specifically quantify multiple different subgenomic SARS-CoV-2 transcripts and distinguish them from transcripts that do not arise from discontinuous transcription at each locus. These assays can be applied to samples from SARS-CoV-2 infected patients to better understand the regulation of SARS-CoV-2 transcription and how different sgRNAs may contribute to viral pathogenesis and clinical disease severity. Twit Text FOAVip Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=33882362 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33882362 #FOAvip English Wikipedia <ref>{{Cite pmid|33882362}}</ref> Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts #MMPMID33882362 Telwatte S; Martin HA; Marczak R; Fozouni P; Vallejo-Gracia A; Kumar GR; Murray V; Lee S; Ott M; Wong JK; Yukl SA Methods 2022[May]; 201 (ä): 15-25 PMID33882362show ga The replication of SARS-CoV-2 and other coronaviruses depends on transcription of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous transcription. Recent studies have aimed to characterize the expression of subgenomic SARS-CoV-2 transcripts in order to investigate their clinical significance. Here, we describe a novel panel of reverse transcription droplet digital PCR (RT-ddPCR) assays designed to specifically quantify multiple different subgenomic SARS-CoV-2 transcripts and distinguish them from transcripts that do not arise from discontinuous transcription at each locus. These assays can be applied to samples from SARS-CoV-2 infected patients to better understand the regulation of SARS-CoV-2 transcription and how different sgRNAs may contribute to viral pathogenesis and clinical disease severity. |*COVID-19/genetics[MESH] |*SARS-CoV-2/genetics[MESH] |Humans[MESH] |Polymerase Chain Reaction[MESH] |RNA, Messenger/genetics[MESH] |RNA, Viral/analysis/genetics[MESH]Novel RT-ddPCR assays for measuring the levels of subgenomic and genom(epmc).pdf DeepDyve Pubget Overpricing