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10.1016/j.bios.2021.113218 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113218 33878591!8052607!33878591     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 184 (ä): 113218 Nephropedia Template TP {{tp|p=33878591|t=2021. Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay |pdf=|usr=}}{{33878591}} gab.com Text Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33878591 #FOAvip Quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples is crucial for early diagnosis and timely medical treatment of coronavirus disease 2019. Here, we describe a digital warm-start CRISPR (dWS-CRISPR) assay for sensitive quantitative detection of SARS-CoV-2 in clinical samples. The dWS-CRISPR assay is initiated at above 50 degrees C and overcomes undesired premature target amplification at room temperature, enabling accurate and reliable digital quantification of SARS-CoV-2. By targeting SARS-CoV-2's nucleoprotein gene, the dWS-CRISPR assay is able to detect down to 5 copies/mul SARS-CoV-2 RNA in the chip. It is clinically validated by quantitatively determining 32 clinical swab samples and three clinical saliva samples. Moreover, it has been demonstrated to directly detect SARS-CoV-2 in heat-treated saliva samples without RNA extraction. Thus, the dWS-CRISPR method, as a sensitive and reliable CRISPR assay, facilitates accurate SARS-CoV-2 detection toward digitized quantification. Twit Text FOAVip Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33878591 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33878591 #FOAvip English Wikipedia <ref>{{Cite pmid|33878591}}</ref> Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay #MMPMID33878591 Ding X; Yin K; Li Z; Sfeir MM; Liu C Biosens Bioelectron 2021[Jul]; 184 (ä): 113218 PMID33878591show ga Quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples is crucial for early diagnosis and timely medical treatment of coronavirus disease 2019. Here, we describe a digital warm-start CRISPR (dWS-CRISPR) assay for sensitive quantitative detection of SARS-CoV-2 in clinical samples. The dWS-CRISPR assay is initiated at above 50 degrees C and overcomes undesired premature target amplification at room temperature, enabling accurate and reliable digital quantification of SARS-CoV-2. By targeting SARS-CoV-2's nucleoprotein gene, the dWS-CRISPR assay is able to detect down to 5 copies/mul SARS-CoV-2 RNA in the chip. It is clinically validated by quantitatively determining 32 clinical swab samples and three clinical saliva samples. Moreover, it has been demonstrated to directly detect SARS-CoV-2 in heat-treated saliva samples without RNA extraction. Thus, the dWS-CRISPR method, as a sensitive and reliable CRISPR assay, facilitates accurate SARS-CoV-2 detection toward digitized quantification. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |CRISPR-Cas Systems/genetics[MESH] |Clustered Regularly Interspaced Short Palindromic Repeats[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral[MESH]Sensitive quantitative detection of SARS-CoV-2 in clinical samples usi(epmc).pdf DeepDyve Pubget Overpricing