Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText.
Kick-your-searchterm to multiple Engines kick-your-query now !>
A dictionary by aggregated review articles of nephrology, medicine and the life sciences
Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

suck abstract from ncbi


10.1038/s42003-021-02001-8

http://scihub22266oqcxt.onion/10.1038/s42003-021-02001-8
suck pdf from google scholar
33875803!8055673!33875803
unlimited free pdf from europmc33875803    free
PDF from PMC    free
html from PMC    free

suck abstract from ncbi

pmid33875803      Commun+Biol 2021 ; 4 (1): 476
Nephropedia Template TP

gab.com Text

Twit Text FOAVip

Twit Text #

English Wikipedia


  • Amplification-free RNA detection with CRISPR-Cas13 #MMPMID33875803
  • Shinoda H; Taguchi Y; Nakagawa R; Makino A; Okazaki S; Nakano M; Muramoto Y; Takahashi C; Takahashi I; Ando J; Noda T; Nureki O; Nishimasu H; Watanabe R
  • Commun Biol 2021[Apr]; 4 (1): 476 PMID33875803show ga
  • CRISPR-based nucleic-acid detection is an emerging technology for molecular diagnostics. However, these methods generally require several hours and could cause amplification errors, due to the pre-amplification of target nucleic acids to enhance the detection sensitivity. Here, we developed a platform that allows "CRISPR-based amplification-free digital RNA detection (SATORI)", by combining CRISPR-Cas13-based RNA detection and microchamber-array technologies. SATORI detected single-stranded RNA targets with maximal sensitivity of ~10 fM in <5 min, with high specificity. Furthermore, the simultaneous use of multiple different guide RNAs enhanced the sensitivity, thereby enabling the detection of the SARS-CoV-2 N-gene RNA at ~5 fM levels. Therefore, we hope SATORI will serve as a powerful class of accurate and rapid diagnostics.
  • |*CRISPR-Cas Systems[MESH]
  • |COVID-19 Nucleic Acid Testing/methods[MESH]
  • |COVID-19/*diagnosis/virology[MESH]
  • |Humans[MESH]
  • |Nucleic Acid Amplification Techniques/*methods[MESH]
  • |RNA, Viral/*genetics/metabolism[MESH]
  • |RNA/*genetics/metabolism[MESH]
  • |Reproducibility of Results[MESH]
  • |SARS-CoV-2/*genetics/physiology[MESH]


  • DeepDyve
  • Pubget Overpricing
  • suck abstract from ncbi

    Linkout box