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10.1007/s42770-021-00498-z

http://scihub22266oqcxt.onion/10.1007/s42770-021-00498-z
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33871824!8053894!33871824
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suck abstract from ncbi


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pmid33871824      Braz+J+Microbiol 2021 ; 52 (3): 1161-1165
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  • Comparative analysis of three point-of-care lateral flow immunoassays for detection of anti-SARS-CoV-2 antibodies: data from 100 healthcare workers in Brazil #MMPMID33871824
  • Conte DD; Carvalho JMA; de Souza Luna LK; Faico-Filho KS; Perosa AH; Bellei N
  • Braz J Microbiol 2021[Sep]; 52 (3): 1161-1165 PMID33871824show ga
  • Since the coronavirus disease 2019 (COVID-19) pandemic, Brazil has the third-highest number of confirmed cases and the second-highest number of recovered patients. SARS-CoV-2 detection by real-time RT-PCR is the gold standard but requires a certified laboratory infrastructure with high-cost equipment and trained personnel. However, for large-scale testing, diagnostics should be fast, cost-effective, widely available, and deployed for the community, such as serological tests based on lateral flow immunoassay (LFIA) for IgM/IgG detection. We evaluated three different commercial point-of-care (POC) LFIAs for anti-SARS-CoV-2 IgM and IgG detection in capillary whole blood of 100 healthcare workers (HCW) from Sao Paulo university hospital previously tested by RT-PCR: (1) COVID-19 IgG/IgM BIO (Bioclin, Brazil), (2) Diagnostic Kit for IgM/IgG Antibody to Coronavirus (SARS-CoV-2) (Livzon, China), and (3) SARS-CoV-2 Antibody Test (Wondfo, China). A total of 84 positives and 16 negatives HCW were tested. The data was also analyzed by the number of days post symptoms (DPS) in three groups: <30 (n=26), 30-59 (n=42), and >59 (n=16). The observed sensibility was 85.71%, 47.62%, and 44.05% for Bioclin, Wondfo, and Livzon, respectively, with a specificity of 100% for all LFIA. Bioclin was more sensitive (p<0.01), regardless of the DPS. Thus, the Bioclin may be used as a POC test to monitor SARS-CoV-2 seroconversion in HCW.
  • |*COVID-19 Serological Testing[MESH]
  • |*Point-of-Care Testing[MESH]
  • |Adult[MESH]
  • |Aged[MESH]
  • |Antibodies, Viral/*blood[MESH]
  • |Brazil/epidemiology[MESH]
  • |COVID-19/*diagnosis/epidemiology[MESH]
  • |Health Personnel[MESH]
  • |Humans[MESH]
  • |Immunoassay[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Immunoglobulin M/blood[MESH]
  • |Middle Aged[MESH]
  • |SARS-CoV-2/immunology/*isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]


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