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10.1007/s40820-021-00620-8

http://scihub22266oqcxt.onion/10.1007/s40820-021-00620-8
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suck abstract from ncbi


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pmid33868761      Nanomicro+Lett 2021 ; 13 (ä): 109
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  • Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection #MMPMID33868761
  • Yang Y; Peng Y; Lin C; Long L; Hu J; He J; Zeng H; Huang Z; Li ZY; Tanemura M; Shi J; Lombardi JR; Luo X
  • Nanomicro Lett 2021[Dec]; 13 (ä): 109 PMID33868761show ga
  • The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 10(6)-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 10(9)-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL(-1) for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40820-021-00620-8.
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