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10.1016/j.bios.2021.113207 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113207 33866136!8020606!33866136     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 183 (ä): 113207 Nephropedia Template TP {{tp|p=33866136|t=2021. Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis |pdf=|usr=}}{{33866136}} gab.com Text Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33866136 #FOAvip Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR or sequencing are tough to implement with limited resources, necessitating the development of accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout for detecting nucleobase and nucleotide sequences without trans-cleavage of reporter molecules. We also demonstrate that FELUDA is 100% accurate in detecting single nucleotide variants (SNVs), including heterozygous carriers, and present a simple web-tool JATAYU to aid end-users. FELUDA is semi-quantitative, can adapt to multiple signal detection platforms, and deploy for versatile applications such as molecular diagnosis during infectious disease outbreaks like COVID-19. Employing a lateral flow readout, FELUDA shows 100% sensitivity and 97% specificity across all ranges of viral loads in clinical samples within 1hr. In combination with RT-RPA and a smartphone application True Outcome Predicted via Strip Evaluation (TOPSE), we present a prototype for FELUDA for CoV-2 detection closer to home. Twit Text FOAVip Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33866136 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33866136 #FOAvip English Wikipedia <ref>{{Cite pmid|33866136}}</ref> Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis #MMPMID33866136 Azhar M; Phutela R; Kumar M; Ansari AH; Rauthan R; Gulati S; Sharma N; Sinha D; Sharma S; Singh S; Acharya S; Sarkar S; Paul D; Kathpalia P; Aich M; Sehgal P; Ranjan G; Bhoyar RC; Singhal K; Lad H; Patra PK; Makharia G; Chandak GR; Pesala B; Chakraborty D; Maiti S Biosens Bioelectron 2021[Jul]; 183 (ä): 113207 PMID33866136show ga Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR or sequencing are tough to implement with limited resources, necessitating the development of accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout for detecting nucleobase and nucleotide sequences without trans-cleavage of reporter molecules. We also demonstrate that FELUDA is 100% accurate in detecting single nucleotide variants (SNVs), including heterozygous carriers, and present a simple web-tool JATAYU to aid end-users. FELUDA is semi-quantitative, can adapt to multiple signal detection platforms, and deploy for versatile applications such as molecular diagnosis during infectious disease outbreaks like COVID-19. Employing a lateral flow readout, FELUDA shows 100% sensitivity and 97% specificity across all ranges of viral loads in clinical samples within 1hr. In combination with RT-RPA and a smartphone application True Outcome Predicted via Strip Evaluation (TOPSE), we present a prototype for FELUDA for CoV-2 detection closer to home. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |COVID-19 Testing[MESH] |Humans[MESH] |RNA, Viral[MESH] |SARS-CoV-2[MESH]Rapid and accurate nucleobase detection using FnCas9 and its applicati(epmc).pdf DeepDyve Pubget Overpricing