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10.1016/j.clim.2021.108730

http://scihub22266oqcxt.onion/10.1016/j.clim.2021.108730
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suck abstract from ncbi


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pmid33865990      Clin+Immunol 2021 ; 226 (ä): 108730
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  • A novel application of delayed-type hipersensitivity reaction to measure cellular immune response in SARS-CoV-2 exposed individuals #MMPMID33865990
  • Barrios Y; Franco A; Sanchez-Machin I; Poza-Guedes P; Gonzalez-Perez R; Matheu V
  • Clin Immunol 2021[May]; 226 (ä): 108730 PMID33865990show ga
  • OBJECTIVE: To understand the anti-virus adaptive immune response occurring during SARS-Cov-2 infection is necessary to have methods to investigate cellular and humoral components. The goal of this study has been to investigate the utility of a specific spike-DTH test using a coronavirus recombinant protein in COVID-19 patients. METHODS: DTH studies were performed by intradermal injection of a commercial recombinant spike protein from SARS-CoV-2 along with conventional serology studies. RESULTS: Fifty-one COVID-19 patients were studied showing 84,3% of concordance with spike-DTH and anti-RBD-IgG. Spike-DTH was superior to identify seven more COVID-19 individuals. A high specificity was found with no positive spike DTH reactions in the non-sick individuals. The skin test also showed more stable results over time while specific anti-RBD-IgG decreased gradually. Clinical severity groups also showed a progressive gradient of larger positive spike-DTH. CONCLUSION: Specific spike DTH test seems to be an easy method to study cell immune response.
  • |Antibodies, Viral/blood[MESH]
  • |COVID-19 Serological Testing/*methods[MESH]
  • |COVID-19/*immunology[MESH]
  • |Female[MESH]
  • |Humans[MESH]
  • |Hypersensitivity, Delayed/*immunology[MESH]
  • |Immunity, Cellular[MESH]
  • |Immunoglobulin G/blood[MESH]
  • |Injections, Intradermal[MESH]
  • |Male[MESH]
  • |Middle Aged[MESH]
  • |Protein Domains[MESH]
  • |Recombinant Proteins[MESH]
  • |SARS-CoV-2/genetics[MESH]


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