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10.1016/j.aca.2021.338390 http://scihub22266oqcxt.onion/10.1016/j.aca.2021.338390 33863409!7973912!33863409     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chim+Acta 2021 ; 1158 (ä): 338390 Nephropedia Template TP {{tp|p=33863409|t=2021. Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2 |pdf=|usr=}}{{33863409}} gab.com Text Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2 JO: Anal Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33863409 #FOAvip The development of rapid, highly sensitive, and selective methods for the diagnosis of infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) should help to prevent the spread of this pandemic virus. In this study, we combined recombinase polymerase amplification (RPA), as a means of isothermal DNA amplification, with an rkDNA-graphene oxide (GO) probe system to allow the rapid detection of SARS-CoV-2 with high sensitivity and selectivity. We used in situ enzymatic synthesis to prepare an rkDNA probe that was complementary to an RPA-amplified sequence of the target N-gene of SARS-CoV-2. The fluorescence of this rkDNA was perfectly quenched in the presence of GO. When the quenched rkDNA-GO system was added to the RPA-amplified sequence of the target SARS-CoV-2, the fluorescence recovered dramatically. The combined RPA/rkDNA-GO system exhibited extremely high selectivity (discrimination factor: 17.2) and sensitivity (LOD = 6.0 aM) for the detection of SARS-CoV-2. The total processing time was only 1.6 h. This combined RPA/rkDNA-GO system appears to be a very efficient and simple method for the point-of-care detection of SARS-CoV-2. Twit Text FOAVip Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2 ????Anal Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33863409 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33863409 #FOAvip English Wikipedia <ref>{{Cite pmid|33863409}}</ref> Combined recombinase polymerase amplification/rkDNA-graphene oxide probing system for detection of SARS-CoV-2 #MMPMID33863409 Choi MH; Lee J; Seo YJ Anal Chim Acta 2021[May]; 1158 (ä): 338390 PMID33863409show ga The development of rapid, highly sensitive, and selective methods for the diagnosis of infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) should help to prevent the spread of this pandemic virus. In this study, we combined recombinase polymerase amplification (RPA), as a means of isothermal DNA amplification, with an rkDNA-graphene oxide (GO) probe system to allow the rapid detection of SARS-CoV-2 with high sensitivity and selectivity. We used in situ enzymatic synthesis to prepare an rkDNA probe that was complementary to an RPA-amplified sequence of the target N-gene of SARS-CoV-2. The fluorescence of this rkDNA was perfectly quenched in the presence of GO. When the quenched rkDNA-GO system was added to the RPA-amplified sequence of the target SARS-CoV-2, the fluorescence recovered dramatically. The combined RPA/rkDNA-GO system exhibited extremely high selectivity (discrimination factor: 17.2) and sensitivity (LOD = 6.0 aM) for the detection of SARS-CoV-2. The total processing time was only 1.6 h. This combined RPA/rkDNA-GO system appears to be a very efficient and simple method for the point-of-care detection of SARS-CoV-2. |*Graphite[MESH] |*Nucleic Acid Amplification Techniques[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |Pandemics[MESH] |Recombinases[MESH] |SARS-CoV-2/*isolation & purification[MESH]Combined recombinase polymerase amplification/rkDNA-graphene oxide pro(epmc).pdf DeepDyve Pubget Overpricing