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10.1021/acs.analchem.1c00192 http://scihub22266oqcxt.onion/10.1021/acs.analchem.1c00192 33861566!ä!33861566 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chem 2021 ; 93 (16): 6456-6462 Nephropedia Template TP {{tp|p=33861566|t=2021. Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR Quantification of Low Viral Load |pdf=|usr=}}{{33861566}} gab.com Text Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR Quantification of Low Viral Load JO: Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33861566 #FOAvip Rapid point-of-care (POC) quantification of low virus RNA load would significantly reduce the turn-around time for the PCR test and help contain a fast-spreading epidemic. Herein, we report a droplet digital PCR (ddPCR) platform that can achieve this sensitivity and rapidity without bulky lab-bound equipment. The key technology is a flattened pipette tip with an elliptical cross-section, which extends a high aspect-ratio microfluidic chip design to pipette scale, for rapid (<5 min) generation of several thousand monodispersed droplets approximately 150 to 350 mum in size with a CV of approximately 2.3%. A block copolymer surfactant (polyoxyalkylene F127) is used to stabilize these large droplets in oil during thermal cycling. At this droplet size and number, positive droplets can be counted by eye or imaged by a smartphone with appropriate illumination/filtering to accurately quantify up to 100 target copies. We demonstrate with 2019 nCoV-PCR assay LODs of 3.8 copies per 20 muL of sample and a dynamic range of 4-100 copies. The ddPCR platform is shown to be inhibitor resistant with spiked saliva samples, suggesting RNA extraction may not be necessary. It represents a rapid 1.5-h POC quantitative PCR test that requires just a pipette equipped with elliptical pipette tip, a commercial portable thermal cycler, a smartphone, and a portable trans-illuminator, without bulky and expensive micropumps and optical detectors that prevent POC application. Twit Text FOAVip Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR Quantification of Low Viral Load ????Anal Chem http://www.kidney.de/pget.php?&tw=1&pmid=33861566 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33861566 #FOAvip English Wikipedia <ref>{{Cite pmid|33861566}}</ref> Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR Quantification of Low Viral Load #MMPMID33861566 Chen L; Yadav V; Zhang C; Huo X; Wang C; Senapati S; Chang HC Anal Chem 2021[Apr]; 93 (16): 6456-6462 PMID33861566show ga Rapid point-of-care (POC) quantification of low virus RNA load would significantly reduce the turn-around time for the PCR test and help contain a fast-spreading epidemic. Herein, we report a droplet digital PCR (ddPCR) platform that can achieve this sensitivity and rapidity without bulky lab-bound equipment. The key technology is a flattened pipette tip with an elliptical cross-section, which extends a high aspect-ratio microfluidic chip design to pipette scale, for rapid (<5 min) generation of several thousand monodispersed droplets approximately 150 to 350 mum in size with a CV of approximately 2.3%. A block copolymer surfactant (polyoxyalkylene F127) is used to stabilize these large droplets in oil during thermal cycling. At this droplet size and number, positive droplets can be counted by eye or imaged by a smartphone with appropriate illumination/filtering to accurately quantify up to 100 target copies. We demonstrate with 2019 nCoV-PCR assay LODs of 3.8 copies per 20 muL of sample and a dynamic range of 4-100 copies. The ddPCR platform is shown to be inhibitor resistant with spiked saliva samples, suggesting RNA extraction may not be necessary. It represents a rapid 1.5-h POC quantitative PCR test that requires just a pipette equipped with elliptical pipette tip, a commercial portable thermal cycler, a smartphone, and a portable trans-illuminator, without bulky and expensive micropumps and optical detectors that prevent POC application. |*COVID-19[MESH] |*Point-of-Care Systems[MESH] |Humans[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH]Elliptical Pipette Generated Large Microdroplets for POC Visual ddPCR (epmc).pdf DeepDyve Pubget Overpricing