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10.3389/fimmu.2021.626308 http://scihub22266oqcxt.onion/10.3389/fimmu.2021.626308 33854501!8040333!33854501     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Immunol 2021 ; 12 (ä): 626308 Nephropedia Template TP {{tp|p=33854501|t=2021. Integrin Activation Enables Sensitive Detection of Functional CD4(+) and CD8(+) T Cells: Application to Characterize SARS-CoV-2 Immunity |pdf=|usr=}}{{33854501}} gab.com Text Integrin Activation Enables Sensitive Detection of Functional CD4(+) and CD8(+) T Cells: Application to Characterize SARS-CoV-2 Immunity JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=33854501 #FOAvip We have previously shown that conformational change in the beta(2)-integrin is a very early activation marker that can be detected with fluorescent multimers of its ligand intercellular adhesion molecule (ICAM)-1 for rapid assessment of antigen-specific CD8(+) T cells. In this study, we describe a modified protocol of this assay for sensitive detection of functional antigen-specific CD4(+) T cells using a monoclonal antibody (clone m24 Ab) specific for the open, high-affinity conformation of the beta(2)-integrin. The kinetics of beta(2)-integrin activation was different on CD4(+) and CD8(+) T cells (several hours vs. few minutes, respectively); however, m24 Ab readily stained both cell types 4-6 h after antigen stimulation. With this protocol, we were able to monitor ex vivo effector and memory CD4(+) and CD8(+) T cells specific for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and hepatitis B virus (HBV) in whole blood or cryopreserved peripheral blood mononuclear cells (PBMCs) of infected or vaccinated individuals. By costaining beta(2)-integrin with m24 and CD154 Abs, we assessed extremely low frequencies of polyfunctional CD4(+) T cell responses. The novel assay used in this study allows very sensitive and simultaneous screening of both CD4(+) and CD8(+) T cell reactivities, with versatile applicability in clinical and vaccination studies. Twit Text FOAVip Integrin Activation Enables Sensitive Detection of Functional CD4(+) and CD8(+) T Cells: Application to Characterize SARS-CoV-2 Immunity ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=33854501 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33854501 #FOAvip English Wikipedia <ref>{{Cite pmid|33854501}}</ref> Integrin Activation Enables Sensitive Detection of Functional CD4(+) and CD8(+) T Cells: Application to Characterize SARS-CoV-2 Immunity #MMPMID33854501 Schollhorn A; Schuhmacher J; Besedovsky L; Fendel R; Jensen ATR; Stevanovic S; Lange T; Rammensee HG; Born J; Gouttefangeas C; Dimitrov S Front Immunol 2021[]; 12 (ä): 626308 PMID33854501show ga We have previously shown that conformational change in the beta(2)-integrin is a very early activation marker that can be detected with fluorescent multimers of its ligand intercellular adhesion molecule (ICAM)-1 for rapid assessment of antigen-specific CD8(+) T cells. In this study, we describe a modified protocol of this assay for sensitive detection of functional antigen-specific CD4(+) T cells using a monoclonal antibody (clone m24 Ab) specific for the open, high-affinity conformation of the beta(2)-integrin. The kinetics of beta(2)-integrin activation was different on CD4(+) and CD8(+) T cells (several hours vs. few minutes, respectively); however, m24 Ab readily stained both cell types 4-6 h after antigen stimulation. With this protocol, we were able to monitor ex vivo effector and memory CD4(+) and CD8(+) T cells specific for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and hepatitis B virus (HBV) in whole blood or cryopreserved peripheral blood mononuclear cells (PBMCs) of infected or vaccinated individuals. By costaining beta(2)-integrin with m24 and CD154 Abs, we assessed extremely low frequencies of polyfunctional CD4(+) T cell responses. The novel assay used in this study allows very sensitive and simultaneous screening of both CD4(+) and CD8(+) T cell reactivities, with versatile applicability in clinical and vaccination studies. |Adult[MESH] |Aged[MESH] |Amino Acid Sequence[MESH] |Binding Sites[MESH] |CD4-Positive T-Lymphocytes/*immunology/*metabolism[MESH] |CD8-Positive T-Lymphocytes/*immunology/*metabolism[MESH] |COVID-19/genetics/immunology/metabolism/virology[MESH] |Carrier Proteins/chemistry[MESH] |Cytokines/metabolism[MESH] |Cytomegalovirus/immunology[MESH] |Epitopes, T-Lymphocyte/chemistry/immunology[MESH] |Female[MESH] |HLA Antigens/chemistry/immunology[MESH] |Host-Pathogen Interactions/genetics/*immunology[MESH] |Humans[MESH] |Immunohistochemistry[MESH] |Immunophenotyping[MESH] |Integrins/genetics/*metabolism[MESH] |Intercellular Adhesion Molecule-1/chemistry/metabolism[MESH] |Lymphocyte Activation/immunology[MESH] |Male[MESH] |Middle Aged[MESH] |Protein Binding[MESH] |Protein Multimerization[MESH] |SARS-CoV-2/immunology[MESH] |T-Cell Antigen Receptor Specificity[MESH]Integrin Activation Enables Sensitive Detection of Functional CD4(+) a(epmc).pdf DeepDyve Pubget Overpricing