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10.1002/adbi.202000154 http://scihub22266oqcxt.onion/10.1002/adbi.202000154 33852178!ä!33852178 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Adv+Biol+(Weinh) 2021 ; 5 (4): e2000154 Nephropedia Template TP {{tp|p=33852178|t=2021. Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens |pdf=|usr=}}{{33852178}} gab.com Text Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens JO: Adv Biol (Weinh) http://www.kidney.de/pget.php?&tw=1&pmid=33852178 #FOAvip Coproduction of multiple proteins at high levels in a single human cell line would be extremely useful for basic research and medical applications. Here, a novel strategy for the stable expression of multiple proteins by integrating the genes into defined transcriptional hotspots in the human genome is presented. As a proof-of-concept, it is shown that EYFP is expressed at similar levels from hotspots and that the EYFP expression increases proportionally with the copy number. It is confirmed that three different fluorescent proteins, encoded by genes integrated at different loci, can be coexpressed at high levels. Further, a stable cell line is generated, producing antigens from different human coronaviruses: MERS-CoV and HCoV-OC43. Antibodies raised against these antigens, which contain human N-glycosylation, show neutralizing activities against both viruses, suggesting that the coexpression system provides a quick and predictable way to produce multiple coronavirus antigens, such as the recent 2019 novel human coronavirus. Twit Text FOAVip Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens ????Adv Biol (Weinh) http://www.kidney.de/pget.php?&tw=1&pmid=33852178 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33852178 #FOAvip English Wikipedia <ref>{{Cite pmid|33852178}}</ref> Efficient Human Cell Coexpression System and Its Application to the Production of Multiple Coronavirus Antigens #MMPMID33852178 Kim C; Jeong YK; Yu J; Shin HJ; Ku KB; Cha HJ; Han JH; Hong SA; Kim BT; Kim SJ; Woo JS; Bae S Adv Biol (Weinh) 2021[Apr]; 5 (4): e2000154 PMID33852178show ga Coproduction of multiple proteins at high levels in a single human cell line would be extremely useful for basic research and medical applications. Here, a novel strategy for the stable expression of multiple proteins by integrating the genes into defined transcriptional hotspots in the human genome is presented. As a proof-of-concept, it is shown that EYFP is expressed at similar levels from hotspots and that the EYFP expression increases proportionally with the copy number. It is confirmed that three different fluorescent proteins, encoded by genes integrated at different loci, can be coexpressed at high levels. Further, a stable cell line is generated, producing antigens from different human coronaviruses: MERS-CoV and HCoV-OC43. Antibodies raised against these antigens, which contain human N-glycosylation, show neutralizing activities against both viruses, suggesting that the coexpression system provides a quick and predictable way to produce multiple coronavirus antigens, such as the recent 2019 novel human coronavirus. |*Antigens, Viral/genetics/immunology[MESH] |*Coronavirus OC43, Human/genetics/immunology[MESH] |*Gene Expression[MESH] |*Middle East Respiratory Syndrome Coronavirus/genetics/immunology[MESH] |Animals[MESH] |Antibodies, Neutralizing/*immunology[MESH] |Antibodies, Viral/*immunology[MESH] |Chlorocebus aethiops[MESH] |Female[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Mice[MESH] |Recombinant Proteins/genetics/immunology[MESH]Efficient Human Cell Coexpression System and Its Application to the Pr(epmc).pdf DeepDyve Pubget Overpricing