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10.7554/eLife.62781 http://scihub22266oqcxt.onion/10.7554/eLife.62781 33843587!8116055!33843587     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2021 ; 10 (ä): ä Nephropedia Template TP {{tp|p=33843587|t=2021. Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node |pdf=|usr=}}{{33843587}} gab.com Text Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=33843587 #FOAvip The detection of foreign antigens in vivo has relied on fluorescent conjugation or indirect read-outs such as antigen presentation. In our studies, we found that these widely used techniques had several technical limitations that have precluded a complete picture of antigen trafficking or retention across lymph node cell types. To address these limitations, we developed a 'molecular tracking device' to follow the distribution, acquisition, and retention of antigen in the lymph node. Utilizing an antigen conjugated to a nuclease-resistant DNA tag, acting as a combined antigen-adjuvant conjugate, and single-cell mRNA sequencing, we quantified antigen abundance in the lymph node. Variable antigen levels enabled the identification of caveolar endocytosis as a mechanism of antigen acquisition or retention in lymphatic endothelial cells. Thus, these molecular tracking devices enable new approaches to study dynamic tissue dissemination of antigen-adjuvant conjugates and identify new mechanisms of antigen acquisition and retention at cellular resolution in vivo. Twit Text FOAVip Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=33843587 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33843587 #FOAvip English Wikipedia <ref>{{Cite pmid|33843587}}</ref> Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node #MMPMID33843587 Walsh SM; Sheridan RM; Lucas ED; Doan TA; Ware BC; Schafer J; Fu R; Burchill MA; Hesselberth JR; Tamburini BAJ Elife 2021[Apr]; 10 (ä): ä PMID33843587show ga The detection of foreign antigens in vivo has relied on fluorescent conjugation or indirect read-outs such as antigen presentation. In our studies, we found that these widely used techniques had several technical limitations that have precluded a complete picture of antigen trafficking or retention across lymph node cell types. To address these limitations, we developed a 'molecular tracking device' to follow the distribution, acquisition, and retention of antigen in the lymph node. Utilizing an antigen conjugated to a nuclease-resistant DNA tag, acting as a combined antigen-adjuvant conjugate, and single-cell mRNA sequencing, we quantified antigen abundance in the lymph node. Variable antigen levels enabled the identification of caveolar endocytosis as a mechanism of antigen acquisition or retention in lymphatic endothelial cells. Thus, these molecular tracking devices enable new approaches to study dynamic tissue dissemination of antigen-adjuvant conjugates and identify new mechanisms of antigen acquisition and retention at cellular resolution in vivo. |*Single-Cell Analysis[MESH] |Animals[MESH] |Antigen Presentation[MESH] |Antigens/genetics/immunology/*metabolism[MESH] |Caveolae/immunology/metabolism[MESH] |Cells, Cultured[MESH] |DNA/genetics/metabolism[MESH] |Dendritic Cells/immunology/metabolism[MESH] |Endocytosis[MESH] |Endothelial Cells/immunology/metabolism[MESH] |Lymph Nodes/immunology/*metabolism[MESH] |Macrophages/immunology/metabolism[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Mice, Transgenic[MESH] |Ovalbumin/genetics/immunology/metabolism[MESH] |Peptide Fragments/genetics/immunology/metabolism[MESH] |Phosphorothioate Oligonucleotides/genetics/metabolism[MESH] |RNA, Messenger/genetics/metabolism[MESH] |Sequence Analysis, RNA[MESH] |Time Factors[MESH] |Tissue Distribution[MESH]Molecular tracking devices quantify antigen distribution and archiving(epmc).pdf DeepDyve Pubget Overpricing