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10.1016/j.celrep.2021.109014 http://scihub22266oqcxt.onion/10.1016/j.celrep.2021.109014 33838744!8015404!33838744     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep 2021 ; 35 (3): 109014 Nephropedia Template TP {{tp|p=33838744|t=2021. Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction |pdf=|usr=}}{{33838744}} gab.com Text Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction JO: Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=33838744 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have been observed in SARS-CoV-2, functional analysis of each mutation of SARS-CoV-2 has been limited by the lack of convenient mutagenesis methods. In this study, we establish a PCR-based, bacterium-free method to generate SARS-CoV-2 infectious clones. Recombinant SARS-CoV-2 could be rescued at high titer with high accuracy after assembling 10 SARS-CoV-2 cDNA fragments by circular polymerase extension reaction (CPER) and transfection of the resulting circular genome into susceptible cells. The construction of infectious clones for reporter viruses and mutant viruses could be completed in two simple steps: introduction of reporter genes or mutations into the desirable DNA fragments ( approximately 5,000 base pairs) by PCR and assembly of the DNA fragments by CPER. This reverse genetics system may potentially advance further understanding of SARS-CoV-2. Twit Text FOAVip Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction ????Cell Rep http://www.kidney.de/pget.php?&tw=1&pmid=33838744 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33838744 #FOAvip English Wikipedia <ref>{{Cite pmid|33838744}}</ref> Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction #MMPMID33838744 Torii S; Ono C; Suzuki R; Morioka Y; Anzai I; Fauzyah Y; Maeda Y; Kamitani W; Fukuhara T; Matsuura Y Cell Rep 2021[Apr]; 35 (3): 109014 PMID33838744show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 (COVID-19). Although multiple mutations have been observed in SARS-CoV-2, functional analysis of each mutation of SARS-CoV-2 has been limited by the lack of convenient mutagenesis methods. In this study, we establish a PCR-based, bacterium-free method to generate SARS-CoV-2 infectious clones. Recombinant SARS-CoV-2 could be rescued at high titer with high accuracy after assembling 10 SARS-CoV-2 cDNA fragments by circular polymerase extension reaction (CPER) and transfection of the resulting circular genome into susceptible cells. The construction of infectious clones for reporter viruses and mutant viruses could be completed in two simple steps: introduction of reporter genes or mutations into the desirable DNA fragments ( approximately 5,000 base pairs) by PCR and assembly of the DNA fragments by CPER. This reverse genetics system may potentially advance further understanding of SARS-CoV-2. |*Reverse Genetics[MESH] |Animals[MESH] |COVID-19/*genetics[MESH] |Cricetinae[MESH] |HEK293 Cells[MESH] |Humans[MESH]Establishment of a reverse genetics system for SARS-CoV-2 using circul(epmc).pdf DeepDyve Pubget Overpricing