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10.7554/eLife.66815 http://scihub22266oqcxt.onion/10.7554/eLife.66815 33835028!8131099!33835028     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Elife 2021 ; 10 (ä): ä Nephropedia Template TP {{tp|p=33835028|t=2021. Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation |pdf=|usr=}}{{33835028}} gab.com Text Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation JO: Elife http://www.kidney.de/pget.php?&tw=1&pmid=33835028 #FOAvip Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 - that expresses serine proteases - prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation. Twit Text FOAVip Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation ????Elife http://www.kidney.de/pget.php?&tw=1&pmid=33835028 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33835028 #FOAvip English Wikipedia <ref>{{Cite pmid|33835028}}</ref> Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation #MMPMID33835028 Lamers MM; Mykytyn AZ; Breugem TI; Wang Y; Wu DC; Riesebosch S; van den Doel PB; Schipper D; Bestebroer T; Wu NC; Haagmans BL Elife 2021[Apr]; 10 (ä): ä PMID33835028show ga Virus propagation methods generally use transformed cell lines to grow viruses from clinical specimens, which may force viruses to rapidly adapt to cell culture conditions, a process facilitated by high viral mutation rates. Upon propagation in VeroE6 cells, SARS-CoV-2 may mutate or delete the multibasic cleavage site (MBCS) in the spike protein. Previously, we showed that the MBCS facilitates serine protease-mediated entry into human airway cells (Mykytyn et al., 2021). Here, we report that propagating SARS-CoV-2 on the human airway cell line Calu-3 - that expresses serine proteases - prevents cell culture adaptations in the MBCS and directly adjacent to the MBCS (S686G). Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation. Thus, in-depth knowledge on the biology of a virus can be used to establish methods to prevent cell culture adaptation. |*Epithelial Cells/cytology/metabolism/virology[MESH] |*Virus Internalization[MESH] |Animals[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Proteolysis[MESH] |Respiratory System/cytology/virology[MESH] |SARS-CoV-2/*physiology[MESH] |Serine Proteases/metabolism[MESH] |Spike Glycoprotein, Coronavirus/*genetics[MESH]Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell cu(epmc).pdf DeepDyve Pubget Overpricing