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Self-sampling of capillary blood for SARS-CoV-2 serology #MMPMID33833246
Brown L; Byrne RL; Fraser A; Owen SI; Cubas-Atienzar AI; Williams CT; Kay GA; Cuevas LE; Fitchett JRA; Fletcher T; Garrod G; Kontogianni K; Krishna S; Menzies S; Planche T; Sainter C; Staines HM; Turtle L; Adams ER
Sci Rep 2021[Apr]; 11 (1): 7754 PMID33833246show ga
Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (>/= 50 microl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen's kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738-1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.
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Sci+Rep 2021 ; 11 (1): 7754
