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10.1371/journal.ppat.1009431 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009431 33831133!8031304!33831133     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33831133&cmd=llinks): Failed to open stream: HTTP request failed! 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High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 |pdf=|usr=}}{{33831133}} gab.com Text High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33831133 #FOAvip Tracking evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within infected individuals will help elucidate coronavirus disease 2019 (COVID-19) pathogenesis and inform use of antiviral interventions. In this study, we developed an approach for sequencing the region encoding the SARS-CoV-2 virion surface proteins from large numbers of individual virus RNA genomes per sample. We applied this approach to the WA-1 reference clinical isolate of SARS-CoV-2 passaged in vitro and to upper respiratory samples from 7 study participants with COVID-19. SARS-CoV-2 genomes from cell culture were diverse, including 18 haplotypes with non-synonymous mutations clustered in the spike NH2-terminal domain (NTD) and furin cleavage site regions. By contrast, cross-sectional analysis of samples from participants with COVID-19 showed fewer virus variants, without structural clustering of mutations. However, longitudinal analysis in one individual revealed 4 virus haplotypes bearing 3 independent mutations in a spike NTD epitope targeted by autologous antibodies. These mutations arose coincident with a 6.2-fold rise in serum binding to spike and a transient increase in virus burden. We conclude that SARS-CoV-2 exhibits a capacity for rapid genetic adaptation that becomes detectable in vivo with the onset of humoral immunity, with the potential to contribute to delayed virologic clearance in the acute setting. Twit Text FOAVip High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33831133 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33831133 #FOAvip English Wikipedia <ref>{{Cite pmid|33831133}}</ref> High-throughput, single-copy sequencing reveals SARS-CoV-2 spike variants coincident with mounting humoral immunity during acute COVID-19 #MMPMID33831133 Ko SH; Bayat Mokhtari E; Mudvari P; Stein S; Stringham CD; Wagner D; Ramelli S; Ramos-Benitez MJ; Strich JR; Davey RT Jr; Zhou T; Misasi J; Kwong PD; Chertow DS; Sullivan NJ; Boritz EA PLoS Pathog 2021[Apr]; 17 (4): e1009431 PMID33831133show ga Tracking evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within infected individuals will help elucidate coronavirus disease 2019 (COVID-19) pathogenesis and inform use of antiviral interventions. In this study, we developed an approach for sequencing the region encoding the SARS-CoV-2 virion surface proteins from large numbers of individual virus RNA genomes per sample. We applied this approach to the WA-1 reference clinical isolate of SARS-CoV-2 passaged in vitro and to upper respiratory samples from 7 study participants with COVID-19. SARS-CoV-2 genomes from cell culture were diverse, including 18 haplotypes with non-synonymous mutations clustered in the spike NH2-terminal domain (NTD) and furin cleavage site regions. By contrast, cross-sectional analysis of samples from participants with COVID-19 showed fewer virus variants, without structural clustering of mutations. However, longitudinal analysis in one individual revealed 4 virus haplotypes bearing 3 independent mutations in a spike NTD epitope targeted by autologous antibodies. These mutations arose coincident with a 6.2-fold rise in serum binding to spike and a transient increase in virus burden. We conclude that SARS-CoV-2 exhibits a capacity for rapid genetic adaptation that becomes detectable in vivo with the onset of humoral immunity, with the potential to contribute to delayed virologic clearance in the acute setting. |*COVID-19/genetics/immunology[MESH] |*Epitopes/genetics/immunology[MESH] |*Immunity, Humoral[MESH] |*Mutation[MESH] |*SARS-CoV-2/genetics/immunology[MESH] |*Spike Glycoprotein, Coronavirus/genetics/immunology[MESH] |Cell Line[MESH] |Female[MESH] |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH]High-throughput, single-copy sequencing reveals SARS-CoV-2 spike varia(epmc).pdf DeepDyve Pubget Overpricing