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10.1073/pnas.2023157118 http://scihub22266oqcxt.onion/10.1073/pnas.2023157118 33827988!8092598!33827988     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2021 ; 118 (17): ä Nephropedia Template TP {{tp|p=33827988|t=2021. ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection |pdf=|usr=}}{{33827988}} gab.com Text ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=33827988 #FOAvip In order to understand the transmission and virulence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is necessary to understand the functions of each of the gene products encoded in the viral genome. One feature of the SARS-CoV-2 genome that is not present in related, common coronaviruses is ORF10, a putative 38-amino acid protein-coding gene. Proteomic studies found that ORF10 binds to an E3 ubiquitin ligase containing Cullin-2, Rbx1, Elongin B, Elongin C, and ZYG11B (CRL2(ZYG11B)). Since CRL2(ZYG11B) mediates protein degradation, one possible role for ORF10 is to "hijack" CRL2(ZYG11B) in order to target cellular, antiviral proteins for ubiquitylation and subsequent proteasomal degradation. Here, we investigated whether ORF10 hijacks CRL2(ZYG11B) or functions in other ways, for example, as an inhibitor or substrate of CRL2(ZYG11B) While we confirm the ORF10-ZYG11B interaction and show that the N terminus of ORF10 is critical for it, we find no evidence that ORF10 is functioning to inhibit or hijack CRL2(ZYG11B) Furthermore, ZYG11B and its paralog ZER1 are dispensable for SARS-CoV-2 infection in cultured cells. We conclude that the interaction between ORF10 and CRL2(ZYG11B) is not relevant for SARS-CoV-2 infection in vitro. Twit Text FOAVip ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=33827988 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33827988 #FOAvip English Wikipedia <ref>{{Cite pmid|33827988}}</ref> ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection #MMPMID33827988 Mena EL; Donahue CJ; Vaites LP; Li J; Rona G; O'Leary C; Lignitto L; Miwatani-Minter B; Paulo JA; Dhabaria A; Ueberheide B; Gygi SP; Pagano M; Harper JW; Davey RA; Elledge SJ Proc Natl Acad Sci U S A 2021[Apr]; 118 (17): ä PMID33827988show ga In order to understand the transmission and virulence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is necessary to understand the functions of each of the gene products encoded in the viral genome. One feature of the SARS-CoV-2 genome that is not present in related, common coronaviruses is ORF10, a putative 38-amino acid protein-coding gene. Proteomic studies found that ORF10 binds to an E3 ubiquitin ligase containing Cullin-2, Rbx1, Elongin B, Elongin C, and ZYG11B (CRL2(ZYG11B)). Since CRL2(ZYG11B) mediates protein degradation, one possible role for ORF10 is to "hijack" CRL2(ZYG11B) in order to target cellular, antiviral proteins for ubiquitylation and subsequent proteasomal degradation. Here, we investigated whether ORF10 hijacks CRL2(ZYG11B) or functions in other ways, for example, as an inhibitor or substrate of CRL2(ZYG11B) While we confirm the ORF10-ZYG11B interaction and show that the N terminus of ORF10 is critical for it, we find no evidence that ORF10 is functioning to inhibit or hijack CRL2(ZYG11B) Furthermore, ZYG11B and its paralog ZER1 are dispensable for SARS-CoV-2 infection in cultured cells. We conclude that the interaction between ORF10 and CRL2(ZYG11B) is not relevant for SARS-CoV-2 infection in vitro. |*Open Reading Frames[MESH] |COVID-19/genetics/*metabolism[MESH] |Cell Cycle Proteins/genetics/*metabolism[MESH] |Cullin Proteins/genetics/*metabolism[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Multiprotein Complexes/genetics/*metabolism[MESH] |SARS-CoV-2/genetics/*metabolism[MESH]ORF10-Cullin-2-ZYG11B complex is not required for SARS-CoV-2 infection(epmc).pdf DeepDyve Pubget Overpricing