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10.1038/s41467-021-21856-3 http://scihub22266oqcxt.onion/10.1038/s41467-021-21856-3 33824342!8024333!33824342     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 2055 Nephropedia Template TP {{tp|p=33824342|t=2021. T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses |pdf=|usr=}}{{33824342}} gab.com Text T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33824342 #FOAvip Identification of protective T cell responses against SARS-CoV-2 requires distinguishing people infected with SARS-CoV-2 from those with cross-reactive immunity to other coronaviruses. Here we show a range of T cell assays that differentially capture immune function to characterise SARS-CoV-2 responses. Strong ex vivo ELISpot and proliferation responses to multiple antigens (including M, NP and ORF3) are found in 168 PCR-confirmed SARS-CoV-2 infected volunteers, but are rare in 119 uninfected volunteers. Highly exposed seronegative healthcare workers with recent COVID-19-compatible illness show T cell response patterns characteristic of infection. By contrast, >90% of convalescent or unexposed people show proliferation and cellular lactate responses to spike subunits S1/S2, indicating pre-existing cross-reactive T cell populations. The detection of T cell responses to SARS-CoV-2 is therefore critically dependent on assay and antigen selection. Memory responses to specific non-spike proteins provide a method to distinguish recent infection from pre-existing immunity in exposed populations. Twit Text FOAVip T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33824342 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33824342 #FOAvip English Wikipedia <ref>{{Cite pmid|33824342}}</ref> T cell assays differentiate clinical and subclinical SARS-CoV-2 infections from cross-reactive antiviral responses #MMPMID33824342 Ogbe A; Kronsteiner B; Skelly DT; Pace M; Brown A; Adland E; Adair K; Akhter HD; Ali M; Ali SE; Angyal A; Ansari MA; Arancibia-Carcamo CV; Brown H; Chinnakannan S; Conlon C; de Lara C; de Silva T; Dold C; Dong T; Donnison T; Eyre D; Flaxman A; Fletcher H; Gardner J; Grist JT; Hackstein CP; Jaruthamsophon K; Jeffery K; Lambe T; Lee L; Li W; Lim N; Matthews PC; Mentzer AJ; Moore SC; Naisbitt DJ; Ogese M; Ogg G; Openshaw P; Pirmohamed M; Pollard AJ; Ramamurthy N; Rongkard P; Rowland-Jones S; Sampson O; Screaton G; Sette A; Stafford L; Thompson C; Thomson PJ; Thwaites R; Vieira V; Weiskopf D; Zacharopoulou P; Turtle L; Klenerman P; Goulder P; Frater J; Barnes E; Dunachie S Nat Commun 2021[Apr]; 12 (1): 2055 PMID33824342show ga Identification of protective T cell responses against SARS-CoV-2 requires distinguishing people infected with SARS-CoV-2 from those with cross-reactive immunity to other coronaviruses. Here we show a range of T cell assays that differentially capture immune function to characterise SARS-CoV-2 responses. Strong ex vivo ELISpot and proliferation responses to multiple antigens (including M, NP and ORF3) are found in 168 PCR-confirmed SARS-CoV-2 infected volunteers, but are rare in 119 uninfected volunteers. Highly exposed seronegative healthcare workers with recent COVID-19-compatible illness show T cell response patterns characteristic of infection. By contrast, >90% of convalescent or unexposed people show proliferation and cellular lactate responses to spike subunits S1/S2, indicating pre-existing cross-reactive T cell populations. The detection of T cell responses to SARS-CoV-2 is therefore critically dependent on assay and antigen selection. Memory responses to specific non-spike proteins provide a method to distinguish recent infection from pre-existing immunity in exposed populations. |Antiviral Agents/*pharmacology[MESH] |CD4-Positive T-Lymphocytes/immunology[MESH] |CD8-Positive T-Lymphocytes/immunology[MESH] |COVID-19/epidemiology/*immunology/*virology[MESH] |Cell Proliferation[MESH] |Cross Reactions/*immunology[MESH] |Cytokines/metabolism[MESH] |HEK293 Cells[MESH] |Health Personnel[MESH] |Humans[MESH] |Immunoassay/*methods[MESH] |Immunoglobulin G/immunology[MESH] |Immunologic Memory[MESH] |Interferon-gamma/metabolism[MESH] |Pandemics[MESH] |Peptides/metabolism[MESH] |SARS-CoV-2/drug effects/*physiology[MESH]T cell assays differentiate clinical and subclinical SARS-CoV-2 infect(epmc).pdf DeepDyve Pubget Overpricing