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10.1016/j.bios.2021.113206 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113206 33823464!8008786!33823464     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 243.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 183 (ä): 113206 Nephropedia Template TP {{tp|p=33823464|t=2021. Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor |pdf=|usr=}}{{33823464}} gab.com Text Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33823464 #FOAvip SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients' samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis. Twit Text FOAVip Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33823464 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33823464 #FOAvip English Wikipedia <ref>{{Cite pmid|33823464}}</ref> Rapid and unamplified identification of COVID-19 with morpholino-modified graphene field-effect transistor nanosensor #MMPMID33823464 Li J; Wu D; Yu Y; Li T; Li K; Xiao MM; Li Y; Zhang ZY; Zhang GJ Biosens Bioelectron 2021[Jul]; 183 (ä): 113206 PMID33823464show ga SARS-CoV-2 RNA is identified as a pivotal player to bolster energizing zones of COVID-19 detection. Herein, we develop a rapid and unamplified nanosensing platform for detection of SARS-CoV-2 RNA in human throat swab specimens. A gold nanoparticle (AuNP)-decorated graphene field-effect transistor (G-FET) sensor was fabricated, after which complementary phosphorodiamidate morpholino oligos (PMO) probe was immobilized on the AuNP surface. This sensor allowed for highly sensitive testing of SARS-CoV-2 RdRp as PMO does not have charges, leading to low background signal. Not only did the method present a low limit of detection in PBS (0.37 fM), throat swab (2.29 fM), and serum (3.99 fM), but also it achieved a rapid response to COVID-19 patients' samples within 2 min. The developed nanosensor was capable of analyzing RNA extracts from 30 real clinical samples. The results show that the sensor could differentiate the healthy people from infected people, which are in high agreement with RT-PCR results (Kappa index = 0.92). Furthermore, a well-defined distinction between SARS-CoV-2 RdRp and SARS-CoV RdRp was also made. Therefore, we believe that this work provides a satisfactory, attractive option for COVID-19 diagnosis. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |*Graphite[MESH] |*Metal Nanoparticles[MESH] |COVID-19 Testing[MESH] |Gold[MESH] |Humans[MESH] |Limit of Detection[MESH] |Morpholinos[MESH] |RNA, Viral[MESH] |SARS-CoV-2[MESH]Rapid and unamplified identification of COVID-19 with morpholino-modif(epmc).pdf DeepDyve Pubget Overpricing