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10.1021/acssensors.1c00235 http://scihub22266oqcxt.onion/10.1021/acssensors.1c00235 33822583!ä!33822583 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Sens 2021 ; 6 (5): 1891-1898 Nephropedia Template TP {{tp|p=33822583|t=2021. Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum |pdf=|usr=}}{{33822583}} gab.com Text Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum JO: ACS Sens http://www.kidney.de/pget.php?&tw=1&pmid=33822583 #FOAvip Rapid and inexpensive serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies are essential to conduct large-scale seroprevalence surveys and can potentially complement nucleic acid or antigen tests at the point of care. During the COVID-19 pandemic, extreme demand for traditional lateral flow tests has stressed manufacturing capacity and supply chains. Motivated by this limitation, we developed a SARS-CoV-2 antibody test using cellulose, an alternative membrane material, and a double-antigen sandwich format. Functionalized SARS-CoV-2 antigens were used as both capture and reporter binders, replacing the anti-human antibodies currently used in lateral flow tests. The test could provide enhanced sensitivity because it labels only antibodies against SARS-CoV-2 and the signal intensity is not diminished due to other human antibodies in serum. Three-dimensional channels in the assay were designed to have consistent flow rates and be easily manufactured by folding wax-printed paper. We demonstrated that this simple, vertical flow, cellulose-based assay could detect SARS-CoV-2 antibodies in clinical samples within 15 min, and the results were consistent with those from a laboratory, bead-based chemiluminescence immunoassay that was granted emergency use approval by the US FDA. Twit Text FOAVip Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum ????ACS Sens http://www.kidney.de/pget.php?&tw=1&pmid=33822583 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33822583 #FOAvip English Wikipedia <ref>{{Cite pmid|33822583}}</ref> Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum #MMPMID33822583 Kim S; Hao Y; Miller EA; Tay DMY; Yee E; Kongsuphol P; Jia H; McBee M; Preiser PR; Sikes HD ACS Sens 2021[May]; 6 (5): 1891-1898 PMID33822583show ga Rapid and inexpensive serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies are essential to conduct large-scale seroprevalence surveys and can potentially complement nucleic acid or antigen tests at the point of care. During the COVID-19 pandemic, extreme demand for traditional lateral flow tests has stressed manufacturing capacity and supply chains. Motivated by this limitation, we developed a SARS-CoV-2 antibody test using cellulose, an alternative membrane material, and a double-antigen sandwich format. Functionalized SARS-CoV-2 antigens were used as both capture and reporter binders, replacing the anti-human antibodies currently used in lateral flow tests. The test could provide enhanced sensitivity because it labels only antibodies against SARS-CoV-2 and the signal intensity is not diminished due to other human antibodies in serum. Three-dimensional channels in the assay were designed to have consistent flow rates and be easily manufactured by folding wax-printed paper. We demonstrated that this simple, vertical flow, cellulose-based assay could detect SARS-CoV-2 antibodies in clinical samples within 15 min, and the results were consistent with those from a laboratory, bead-based chemiluminescence immunoassay that was granted emergency use approval by the US FDA. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Antibodies, Viral[MESH] |Cellulose[MESH] |Humans[MESH] |Immunoassay[MESH] |Pandemics[MESH] |Sensitivity and Specificity[MESH]Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection(epmc).pdf DeepDyve Pubget Overpricing