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10.2144/btn-2020-0159 http://scihub22266oqcxt.onion/10.2144/btn-2020-0159 33820475!8023013!33820475     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechniques 2021 ; 70 (4): 218-225 Nephropedia Template TP {{tp|p=33820475|t=2021. Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification |pdf=|usr=}}{{33820475}} gab.com Text Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification JO: Biotechniques http://www.kidney.de/pget.php?&tw=1&pmid=33820475 #FOAvip Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection. Twit Text FOAVip Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification ????Biotechniques http://www.kidney.de/pget.php?&tw=1&pmid=33820475 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33820475 #FOAvip English Wikipedia <ref>{{Cite pmid|33820475}}</ref> Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification #MMPMID33820475 Reynes B; Serra F; Palou A Biotechniques 2021[Apr]; 70 (4): 218-225 PMID33820475show ga Evaluation of the performance of a new set of primers defined from the ORF1ab sequence, and its combination with a previously published set of primers from the N sequence, to detect SARS-CoV-2 RNA by the loop-mediated isothermal amplification technique is presented. The ORF1ab primer set enables visual detection of SARS-CoV-2 RNA in 16 min. In addition, a simultaneous reaction with both ORF1ab and N primers allows for higher sensitivity of detection, particularly when low numbers of copies are present (250 viral RNA copies). Further, the protocol is able to detect viral RNA in saliva samples. The procedure reported could be easily implemented in the generation of a new and sensitive rapid point-of care device for SARS-CoV-2 RNA visual detection. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Colorimetry/*methods[MESH] |DNA Primers[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Polyproteins/genetics[MESH] |SARS-CoV-2/*genetics[MESH]Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated iso(epmc).pdf DeepDyve Pubget Overpricing