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10.1016/j.nmni.2021.100874 http://scihub22266oqcxt.onion/10.1016/j.nmni.2021.100874 33815807!8010344!33815807     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       New+Microbes+New+Infect 2021 ; 41 (ä): 100874 Nephropedia Template TP {{tp|p=33815807|t=2021. TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019 |pdf=|usr=}}{{33815807}} gab.com Text TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019 JO: New Microbes New Infect http://www.kidney.de/pget.php?&tw=1&pmid=33815807 #FOAvip Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of RNA extraction methods. RNA extraction was performed using three different procedures (TRIzol, QIAamp, VMT-TRIzol) from nine positive SARS-CoV-2 samples. SARS-CoV-2 was detected by real-time reverse transcriptase PCR (RT-PCR) using a detection kit for SARS-CoV-2 (Sun Yat-sen University). Compared to RT-PCR results, there were no discernible differences in detection rates when comparing the three different extraction procedures. On the basis of these results, the use of TRIzol as a transport medium and RNA extraction method for SARS-CoV-2 detection may be a helpful alternative for laboratories facing shortages of commercial testing kits. Twit Text FOAVip TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019 ????New Microbes New Infect http://www.kidney.de/pget.php?&tw=1&pmid=33815807 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33815807 #FOAvip English Wikipedia <ref>{{Cite pmid|33815807}}</ref> TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019 #MMPMID33815807 Amirouche A; Ait-Ali D; Nouri H; Boudrahme-Hannou L; Tliba S; Ghidouche A; Bitam I New Microbes New Infect 2021[May]; 41 (ä): 100874 PMID33815807show ga Diagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared the sensitivity of RNA extraction methods. RNA extraction was performed using three different procedures (TRIzol, QIAamp, VMT-TRIzol) from nine positive SARS-CoV-2 samples. SARS-CoV-2 was detected by real-time reverse transcriptase PCR (RT-PCR) using a detection kit for SARS-CoV-2 (Sun Yat-sen University). Compared to RT-PCR results, there were no discernible differences in detection rates when comparing the three different extraction procedures. On the basis of these results, the use of TRIzol as a transport medium and RNA extraction method for SARS-CoV-2 detection may be a helpful alternative for laboratories facing shortages of commercial testing kits. äTRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of c(epmc).pdf DeepDyve Pubget Overpricing