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10.1016/j.jviromet.2021.114146 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114146 33812944!8015351!33812944     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 293 (ä): 114146 Nephropedia Template TP {{tp|p=33812944|t=2021. A novel method for conserved sequence extraction with prospective mutation prediction for SARS-CoV-2 PCR primer design |pdf=|usr=}}{{33812944}} gab.com Text A novel method for conserved sequence extraction with prospective mutation prediction for SARS-CoV-2 PCR primer design JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33812944 #FOAvip While the whole genomic sequence of SARS-CoV-2 had been revealed, it was also demonstrated that the genome of SARS-CoV-2 exhibits identity with the genome of SARS-CoV and MERS-CoV with ratios of 80 % and 50 % respectively. In the light of SARS-CoV-2 infection and mortality data, diagnosis and treatment of COVID-19 came into prominence around the world. As such many RT-PCR kits have been developed by biotechnology scientists. However viruses are fast mutating organisms and in order to increase accuracy, feasibility in long term and avoid the off target results of RT-PCR assays, regions of viral genome with low mutation rate and designing of primers targeting these regions are quite important. In this scope, we are presenting a novel algorithm that could be used for finding low mutation rate regions of SARS-CoV-2 and primers that were designed according to findings from our algorithm in this study. Twit Text FOAVip A novel method for conserved sequence extraction with prospective mutation prediction for SARS-CoV-2 PCR primer design ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33812944 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33812944 #FOAvip English Wikipedia <ref>{{Cite pmid|33812944}}</ref> A novel method for conserved sequence extraction with prospective mutation prediction for SARS-CoV-2 PCR primer design #MMPMID33812944 Portakal SH; Kanat B; Sayan M; Berber B; Doluca O J Virol Methods 2021[Jul]; 293 (ä): 114146 PMID33812944show ga While the whole genomic sequence of SARS-CoV-2 had been revealed, it was also demonstrated that the genome of SARS-CoV-2 exhibits identity with the genome of SARS-CoV and MERS-CoV with ratios of 80 % and 50 % respectively. In the light of SARS-CoV-2 infection and mortality data, diagnosis and treatment of COVID-19 came into prominence around the world. As such many RT-PCR kits have been developed by biotechnology scientists. However viruses are fast mutating organisms and in order to increase accuracy, feasibility in long term and avoid the off target results of RT-PCR assays, regions of viral genome with low mutation rate and designing of primers targeting these regions are quite important. In this scope, we are presenting a novel algorithm that could be used for finding low mutation rate regions of SARS-CoV-2 and primers that were designed according to findings from our algorithm in this study. |*DNA Primers[MESH] |*Mutation[MESH] |Algorithms[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |Prospective Studies[MESH] |SARS-CoV-2/*genetics[MESH]A novel method for conserved sequence extraction with prospective muta(epmc).pdf DeepDyve Pubget Overpricing