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10.3390/v13040562 http://scihub22266oqcxt.onion/10.3390/v13040562 33810401!8066162!33810401     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2021 ; 13 (4): ä Nephropedia Template TP {{tp|p=33810401|t=2021. Effect of Inactivation Methods on SARS-CoV-2 Virion Protein and Structure |pdf=|usr=}}{{33810401}} gab.com Text Effect of Inactivation Methods on SARS-CoV-2 Virion Protein and Structure JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33810401 #FOAvip The risk posed by Severe Acute Respiratory Syndrome Coronavirus -2 (SARS-CoV-2) dictates that live-virus research is conducted in a biosafety level 3 (BSL3) facility. Working with SARS-CoV-2 at lower biosafety levels can expedite research yet requires the virus to be fully inactivated. In this study, we validated and compared two protocols for inactivating SARS-CoV-2: heat treatment and ultraviolet irradiation. The two methods were optimized to render the virus completely incapable of infection while limiting the destructive effects of inactivation. We observed that 15 min of incubation at 65 degrees C completely inactivates high titer viral stocks. Complete inactivation was also achieved with minimal amounts of UV power (70,000 microJ/cm(2)), which is 100-fold less power than comparable studies. Once validated, the two methods were then compared for viral RNA quantification, virion purification, and antibody detection assays. We observed that UV irradiation resulted in a 2-log reduction of detectable genomes compared to heat inactivation. Protein yield following virion enrichment was equivalent for all inactivation conditions, but the quality of resulting viral proteins and virions were differentially impacted depending on inactivation method and time. Here, we outline the strengths and weaknesses of each method so that investigators might choose the one which best meets their research goals. Twit Text FOAVip Effect of Inactivation Methods on SARS-CoV-2 Virion Protein and Structure ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=33810401 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33810401 #FOAvip English Wikipedia <ref>{{Cite pmid|33810401}}</ref> Effect of Inactivation Methods on SARS-CoV-2 Virion Protein and Structure #MMPMID33810401 Loveday EK; Hain KS; Kochetkova I; Hedges JF; Robison A; Snyder DT; Brumfield SK; Young MJ; Jutila MA; Chang CB; Taylor MP Viruses 2021[Mar]; 13 (4): ä PMID33810401show ga The risk posed by Severe Acute Respiratory Syndrome Coronavirus -2 (SARS-CoV-2) dictates that live-virus research is conducted in a biosafety level 3 (BSL3) facility. Working with SARS-CoV-2 at lower biosafety levels can expedite research yet requires the virus to be fully inactivated. In this study, we validated and compared two protocols for inactivating SARS-CoV-2: heat treatment and ultraviolet irradiation. The two methods were optimized to render the virus completely incapable of infection while limiting the destructive effects of inactivation. We observed that 15 min of incubation at 65 degrees C completely inactivates high titer viral stocks. Complete inactivation was also achieved with minimal amounts of UV power (70,000 microJ/cm(2)), which is 100-fold less power than comparable studies. Once validated, the two methods were then compared for viral RNA quantification, virion purification, and antibody detection assays. We observed that UV irradiation resulted in a 2-log reduction of detectable genomes compared to heat inactivation. Protein yield following virion enrichment was equivalent for all inactivation conditions, but the quality of resulting viral proteins and virions were differentially impacted depending on inactivation method and time. Here, we outline the strengths and weaknesses of each method so that investigators might choose the one which best meets their research goals. |COVID-19/*virology[MESH] |Disinfection/instrumentation/*methods[MESH] |Hot Temperature[MESH] |Humans[MESH] |SARS-CoV-2/chemistry/genetics/physiology/*radiation effects[MESH] |Ultraviolet Rays[MESH] |Viral Proteins/genetics/metabolism[MESH] |Virion/chemistry/genetics/physiology/*radiation effects[MESH]Effect of Inactivation Methods on SARS-CoV-2 Virion Protein and Struct(epmc).pdf DeepDyve Pubget Overpricing