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10.3390/nano11030807 http://scihub22266oqcxt.onion/10.3390/nano11030807 33809836!8004173!33809836     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nanomaterials+(Basel) 2021 ; 11 (3): ä Nephropedia Template TP {{tp|p=33809836|t=2021. A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion |pdf=|usr=}}{{33809836}} gab.com Text A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion JO: Nanomaterials (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33809836 #FOAvip High-throughput detection strategies for antibodies against SARS-CoV-2 in patients recovering from COVID-19, or in vaccinated individuals, are urgently required during this ongoing pandemic. Serological assays are the most widely used method to measure antibody responses in patients. However, most of the current methods lack the speed, stability, sensitivity, and specificity to be selected as a test for worldwide serosurveys. Here, we demonstrate a novel NanoBiT-based serological assay for fast and sensitive detection of SARS-CoV-2 RBD-specific antibodies in sera of COVID-19 patients. This assay can be done in high-throughput manner at 384 samples per hour and only requires a minimum of 5 muL of serum or 10 ng of antibody. The stability of our NanoBiT reporter in various temperatures (4-42 degrees C) and pH (4-12) settings suggests the assay will be able to withstand imperfect shipping and handling conditions for worldwide seroepidemiologic surveillance in the post-vaccination period of the pandemic. Our newly developed rapid assay is highly accessible and may facilitate a more cost-effective solution for seroconversion screening as vaccination efforts progress. Twit Text FOAVip A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion ????Nanomaterials (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=33809836 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33809836 #FOAvip English Wikipedia <ref>{{Cite pmid|33809836}}</ref> A High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 Seroconversion #MMPMID33809836 Azad T; Rezaei R; Singaravelu R; Jamieson TR; Crupi MJF; Surendran A; Poutou J; Taklifi P; Cowan J; Cameron DW; Ilkow CS Nanomaterials (Basel) 2021[Mar]; 11 (3): ä PMID33809836show ga High-throughput detection strategies for antibodies against SARS-CoV-2 in patients recovering from COVID-19, or in vaccinated individuals, are urgently required during this ongoing pandemic. Serological assays are the most widely used method to measure antibody responses in patients. However, most of the current methods lack the speed, stability, sensitivity, and specificity to be selected as a test for worldwide serosurveys. Here, we demonstrate a novel NanoBiT-based serological assay for fast and sensitive detection of SARS-CoV-2 RBD-specific antibodies in sera of COVID-19 patients. This assay can be done in high-throughput manner at 384 samples per hour and only requires a minimum of 5 muL of serum or 10 ng of antibody. The stability of our NanoBiT reporter in various temperatures (4-42 degrees C) and pH (4-12) settings suggests the assay will be able to withstand imperfect shipping and handling conditions for worldwide seroepidemiologic surveillance in the post-vaccination period of the pandemic. Our newly developed rapid assay is highly accessible and may facilitate a more cost-effective solution for seroconversion screening as vaccination efforts progress. äA High-Throughput NanoBiT-Based Serological Assay Detects SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing