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10.3390/biology10030208

http://scihub22266oqcxt.onion/10.3390/biology10030208
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suck abstract from ncbi


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pmid33801808      Biology+(Basel) 2021 ; 10 (3): ä
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  • Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2 #MMPMID33801808
  • Santiago L; Uranga-Murillo I; Arias M; Gonzalez-Ramirez AM; Macias-Leon J; Moreo E; Redrado S; Garcia-Garcia A; Taleb V; Lira-Navarrete E; Hurtado-Guerrero R; Aguilo N; Del Mar Encabo-Berzosa M; Hidalgo S; Galvez EM; Ramirez-Labrada A; de Miguel D; Benito R; Miranda P; Fernandez A; Domingo JM; Serrano L; Yuste C; Villanueva-Saz S; Pano-Pardo JR; Pardo J
  • Biology (Basel) 2021[Mar]; 10 (3): ä PMID33801808show ga
  • Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T- and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2.
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